文献类型：期刊文献

中文题名：敦煌医方瞿麦汤对草酸钙型肾结石模型大鼠肾功能的影响及可能机制

英文题名：The effect and possible mechanism of Dunhuang Yifang,Qu Mai decoction,on the renal function of rats with calcium oxalate nephrolithiasis

作者：安方玉[1,2];颜春鲁[1,2];刘永琦[2];赵文坤[3];牛彦强[4];刘明月[3];辛阳[3];杨晓蓉[4]

第一作者：安方玉

机构：[1]甘肃中医药大学教学实验实训中心,兰州730000;[2]甘肃中医药大学敦煌医学与转化教育部重点实验室,兰州730000;[3]甘肃中医药大学公共卫生学院,兰州730000;[4]甘肃中医药大学临床医学院,兰州730000

第一机构：甘肃中医药大学教学实验实训中心

年份：2019

卷号：46

期号：3

起止页码：194

中文期刊名：国际药学研究杂志

外文期刊名：Journal of International Pharmaceutical Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；

基金：敦煌医学与转化教育部重点实验室开放基金项目(DHYX17-08,DHYX17-09);甘肃省高校大学生就业创业能力提升工程项目(6-1);甘肃中医药大学教学研究与教学改革项目(ZH-201606)

语种：中文

中文关键词：敦煌医方瞿麦汤;草酸钙型肾结石;单核细胞趋化蛋白-1(MCP-1);骨桥蛋白(OPN);中性粒细胞明胶酶相关脂质运载蛋白(NGAL)

外文关键词：Dunhuang Yifang Qu Mai decoction;calcium oxalate nephrolithiasis;monocyte chemoattractant protein-1(MCP-1);osteopontin(OPN);neutrophil gelatinase-associated lipocalin(NGAL)

摘要：目的探讨敦煌医方瞿麦汤对草酸钙型肾结石模型大鼠肾功能的影响及可能机制。方法雄性SD大鼠60只,随机分为正常对照组,模型组,肾石通颗粒组,以及瞿麦汤5、10、20 g/(kg·d)剂量组,每组10只。给药组和模型组采用1%乙二醇+2%氯化铵的混合液每日2 ml灌胃,连续28 d;同时给予药物干预,肾石通颗粒组给予肾石通颗粒2.8 g/(kg·d)灌胃治疗,瞿麦汤组按5、10、20 g/(kg·d)灌胃治疗,正常对照组和模型组大鼠给予等体积蒸馏水灌胃,每日1次,连续4周。末次给药24 h后,称量各组大鼠体质量,计算肾指数;采用HE染色法观察肾组织的病理形态变化;采用ELISA法检测各组大鼠尿液中单核细胞趋化蛋白-1(MCP-1)、骨桥蛋白(OPN)和中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的含量;采用比色分析法检测各组大鼠血清中尿素氮(BUN)、肌酐(Cr)含量和肾组织总抗氧化能力(T-AOC)的活性。结果与正常对照组比较,模型组大鼠体质量显著降低、肾组织T-AOC活性明显下降(P<0.05),肾指数明显升高,尿液MCP-1、OPN、NGAL含量和血清BUN和Cr含量均显著升高(P<0.05)。与模型组比较,各治疗组体质量明显升高(P<0.05),肾指数明显降低(P<0.05),血清BUN和尿液MCP-1、OPN、NGAL含量均明显降低(P<0.05);瞿麦汤10和20 g/(kg·d)剂量组和肾石通颗粒组血清Cr含量明显降低(P<0.05);瞿麦汤20 g/(kg·d)剂量组和肾石通颗粒组肾组织T-AOC活性明显升高(P<0.05)。结论瞿麦汤可显著改善肾草酸钙结石模型大鼠的肾功能,其作用机制可能与下调MCP-1、OPN、NGAL等蛋白的表达和减少BUN、Cr的含量有关。
Objective To investigate the effect and possible mechanism of Dunhuang Yifang,Qu Mai decoction,on the renal function of rats with calcium oxalate nephrolithiasis. Methods Sixty male SD rats were randomly divided into the normal control group(equal volume distilled water),model group(equal volume distilled water),Shenshitong granule group 2.8 g/(kg·d)and the Qu Mai decoction 5,10,and 20 g/(kg·d)groups,with 10 rats in each group. Except for the normal control group,rats in the other groups were given 1% ethylene glycol and 2% ammonium chloride(2% NH4 Cl)to establish calcium oxalate nephrolithiasis model,and the rats were orally administered the corresponding drugs for 4 weeks. After the last administration of the drugs,the body mass was weighed,the kidney index was caculated,the pathomorphological changes in the renal tissues were examined by HE staining,the urine monocyte chemoattractant protein-1(MCP-1),osteopontin(OPN)and neutrophil gelatinase-associated lipocalin(NGAL)level was determined by the ELISA method,and the serum urea nitrogen(RUN)and creatinine(Cr)level as well as the T-AOC activity of kidney tissues were determined by the colorimetry. Results Compared with the normal control group,the body mass and the kidney T-AOC activity decreased significantly(P<0.05),while the kidney index,the urine MCP-1,OPN and NGAL level as well as the serum BUN and Cr level increased,all significantly(all P<0.05),in the model group. Compared with the model group,the body mass increased significantly(P<0.05),while the kidney index and the urine MCP-1,OPN and NGAL level as well the serum BUN level decreased,all significantly(all P<0.05),in the drug-administered groups. Meanwhile,the serum Cr level significantly decreased in the Qu Mai decoction 10 and 20 g/(kg·d)and the Shenshitong granule groups(all P<0.05),while the kidney T-AOC activity significantly increased in the Qu Mai decoction 20 g/(kg·d)and the Shenshitong granule groups(both P<0.05). Conclusion The Qu Mai decoction could effectively improve the kidney function(renal function)of the calcium oxalate nephrolithiasis model rats,and the mechanism may be related to the down regulation of MCP-1,OPN and NGAL protein expression as well as the reduction of serum BUN and Cr level.