文献类型：期刊文献

中文题名：红芪等4种中药多糖对环磷酰胺所致免疫低下小鼠模型的免疫调节作用对比研究

英文题名：Comparative study of the immunomodulatory effects of Hedysarum polybotrys etc.four species traditional Chinese medicine polysaccharides on immunocompromised mice model made by cyclophosphamide

作者：邵晶[1,2];杜丽东[1,3];吴国泰[1,3];杨志军[1,2];郭玫[1,2]

第一作者：邵晶

机构：[1]甘肃中医药大学药学院;[2]甘肃中医药大学甘肃省高校中(藏)药化学与质量研究省级重点实验室;[3]甘肃中医药大学甘肃省中药药理与毒理学重点实验室

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：33

期号：21

起止页码：2175

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：甘肃省自然科学基金资助项目(17JR5RA163);甘肃省高等学校基本科研业务费基金资助项目(2013-1);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金资助项目(zzy-2014-04);甘肃省教育厅科研基金资助项目(2013A-085)

语种：中文

中文关键词：红芪多糖;黄芪多糖;党参多糖;当归多糖;免疫调节

外文关键词：Hedysarum polybotrys polysaccharides; Astragalus polysaccharides; Codonopsis pilosula polysaccharides;Angelica polysaccharides ; immunoregulation

摘要：目的对比研究红芪等4种中药多糖对环磷酰胺所致免疫低下小鼠模型的免疫调节作用。方法用环磷酰胺40 mg·kg^(-1)制备免疫低下小鼠模型。按照体重将昆明种小鼠随机分为7组:正常组、模型组、对照组、红芪多糖组、黄芪多糖组、党参多糖组、当归多糖组。对照组灌胃参芪颗粒5 g·kg^(-1),其他各组分别灌胃相应的受试样品2 g·kg^(-1),正常组和模型组灌胃等量0.9%NaCl,每天给药1次,连续15 d。称重法计算小鼠脏器指数;腹腔注射5%鸡红细胞悬液测定腹腔巨噬细胞吞噬百分率及吞噬指数;50%二硝基氯苯丙酮溶液致迟发型超敏反应测定OD值;5%鸡红细胞悬液(CRBC)致敏测定血清溶血素OD值;尾静脉注射印度墨汁测定网状内皮系统吞噬指数及吞噬系数;用紫外分光光度法测定多糖含量。结果与正常组比较,模型组各指标差异均有统计学意义(均P<0.01)。给药后,模型组与红芪多糖组、黄芪多糖组、党参多糖组、当归多糖组的胸腺指数(g·kg^(-1))分别是2.55±0.88,1.04±0.74,2.00±0.89,2.21±0.95,1.92±0.43,1.67±0.41,与模型组比较,红芪多糖组、黄芪多糖组、党参多糖组差异均有统计学意义(均P<0.05)。这6组的腹腔巨噬细胞吞噬指数分别是1.31±0.18,0.51±0.12,1.12±0.13,1.19±0.09,0.88±0.25,0.72±0.07,与模型组比较,4种多糖组差异均有统计学意义(均P<0.01);与当归多糖组比较,红芪多糖组、黄芪多糖组的差异均有统计学意义(P<0.05或P<0.01)。这6组的网状内皮系统吞噬系数分别是6.60±0.94,0.99±0.83,0.32±1.48,7.28±2.13,5.29±1.97,5.93±1.75,与模型组比较,红芪多糖组、黄芪多糖组差异均有统计学意义(P<0.05或P<0.01)。这6组的迟发型超敏反应OD值分别为0.53±0.16,0.23±0.09,0.36±0.09,0.38±0.12,0.29±0.10,0.33±0.11;这6组的血清溶血素OD值分别为0.60±0.12,0.43±0.10,0.53±0.08,0.54±0.11,0.51±0.11,0.52±0.09;与模型组比较,红芪多糖、黄芪多糖、当归多糖迟发型超敏反应OD值和血清溶血素OD值差异均有统计学意义(P<0.05或P<0.01)。结论这4种中药多糖均具有提高小鼠非特异性免疫功能、特异性体液免疫功能和细胞免疫功能的作用。但红芪多糖与黄芪多糖对各免疫指标的调节要更全面且显著一些。
Objective To comparative study of the immunomodulatory effects of Hedysarum polybotrys polysaccharides（ HPS） etc. four species TCM polysaccharides on immunocompromised mice model made by cyclophosphamide. Methods Low-immunity model mice made bycyclophosphamide 40 mg·kg^-1. The KM mice were divided into seven group： normal,model,control,experimental-HPS,experimental-Astragalus polysaccharides（ ASPS）,experimental-Codonopsis pilosula polysaccharides（ CPS）and experimental-Angelica polysaccharides（ ANPS） groups randomly. The control group was administrated Shenqi particle 5 g·kg^-1. The other tested groups were administrated the corresponding test samples 2 g·kg^-1. The normal and model group were administrated the same quantity of normal saline. Give the medicine 1 time each day for 15 days.The visceral index was counted by weighted. The peritoneal macrophage phagocytic count by intraperitoneal injection5% chicken red blood-cell were meaeured（ OD value）. The serum hemolysin OD through delayed type hypersensitivity maded by 50% dinitrochlorobenzene. The reticuloendothelial system phagocytic count were meaeured by caudal vein injection carbon inkl. Results Compared with the normal,the difference of all indexs of model were statistically significant（ all P〈0. 01）. After dosing,the thymus indexs of model,control,experimental-HPS,experimental-ASPS,experimental-CPS and experimental-ANPS groups were 2. 55 ± 0. 88,1. 04 ± 0. 74,2. 00 ± 0. 89,2. 21 ± 0. 95,1. 92 ± 0. 43,1. 67 ± 0. 41. Compared with the model,difference of experimental-HPS,experimental-ASPS and experimental-CPS were statistically significant（ all P〈0. 05）. The phagocytic counts of mouse peritoneal macrophages of the six groups were 1. 31 ± 0. 18,0. 51 ± 0. 12,1. 12 ± 0. 13,1. 19 ± 0. 09,0. 88 ± 0. 25,0. 72 ± 0. 07.Compared with the model,difference of four species polysaccharides were all statistically significant（ all P〈0. 01）.Compared with the experimental-ANPS,difference of experimental-HPS and experimental-ASPS were statistically significant（ P〈0. 05,P〈0. 01）. The phagocytic coefficient of reticuloendothelial system phagocytosis of the six groups were 6. 60 ± 0. 94,0. 99 ± 0. 83,0. 32 ± 1. 48,7. 28 ± 2. 13,5. 29 ± 1. 97,5. 93 ± 1. 75. Compared with the model,difference of experimental-HPS and experimental-ASPS were statistically significant（ P〈0. 05 or P〈0. 01）. The delayed type hypersensitivity OD of the six groups were 0. 53 ± 0. 16,0. 23 ± 0. 09,0. 36 ± 0. 09,0. 38 ± 0. 12,0. 29 ± 0. 10,0. 33 ± 0. 11. The serum hemolysin OD of the six groups were 0. 60 ± 0. 12,0. 43 ± 0. 10,0. 53 ± 0. 08,0. 54 ± 0. 11, 0. 51 ± 0. 11, 0. 52 ± 0. 09. Compared with the model, difference of experimental-HPS,experimental-ASPS and experimental-ANPS were statistically significant（ P〈0. 05 or P〈0. 01）. Conclusion These four kinds of polysaccharides have the function of enhancing nonspecific immunity function,specific humoral immunity and cellular immune function in mice. HPS and ASPS were more comprehensive and significant for the regulation of immune indexes than CPS and ANPS.