文献类型：期刊文献

中文题名：提高春小麦幼胚离体培养中愈伤组织诱导及分化效率的研究

英文题名：Improving Callus Induction and Differentiation Efficiency of In-vitro Immature Embryo of Spring Wheat (Triticum aestivum L.)

作者：张金林[1];石明辉[2];许瑞[3];李唯[4];王锁民[1]

第一作者：张金林

机构：[1]兰州大学草地农业科技学院农业部草地农业生态系统学重点开放实验室,兰州730020;[2]甘肃省酒泉市农科所,酒泉735000;[3]甘肃中医学院,兰州730020;[4]甘肃农业大学生命科学技术学院,兰州730070

第一机构：兰州大学草地农业科技学院农业部草地农业生态系统学重点开放实验室,兰州730020

年份：2007

卷号：23

期号：4

起止页码：49

中文期刊名：中国农学通报

外文期刊名：Chinese Agricultural Science Bulletin

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：兰州大学交叉学科青年创新研究基金"利用液泡Na+积累功能基因转化紫花苜蓿的研究"(LZU200516);2004年度甘肃省农业生物技术研究与应用开发项目"抗旱耐盐转基因豆科牧草新品种培育"(GNSW-22004207);农业部草地农业生态系统重点开放实验室2004年开放课题基金(2004-014)。

语种：中文

中文关键词：春小麦;愈伤组织诱导;芽分化;植株再生;效率

外文关键词：Spring wheat, Callus induction, Differentiation, Regeneration, Efficiency

摘要：以甘肃省7个春小麦品种为试验材料,探讨了接种方式、基因型、胚龄、植物生长调节剂等因素对幼胚培养中愈伤组织诱导及分化的影响。结果表明:供试春小麦品种出愈率不受盾片放置方向的影响,而盾片向上放置的分化率明显大于盾片向下放置的;春小麦幼胚离体培养的有效胚龄期为14～20d,最适宜的胚龄期为16d;基因型对愈伤组织诱导无影响,但对愈伤组织再生能力有极其重要的影响;随2,4-D浓度的增加春小麦幼胚分化率呈单峰曲线变化,且品种间差异很大,对大多数品种而言,适宜的2,4-D浓度为1～6mg/L。0.2mg/LNAA和0.2mg/LIAA能有效地诱导芽快速生根,生根率分别达到83.8%和55.1%。
Effects of inoculation direction, genotypes, embryo ages and plant growth regulators on callus induction and differentiation efficiency of in-vitro immature embryo of seven local spring wheat cultivars of Gansu were investigated in this research. The results indicated： inoculation direction had no significant effects on callus induction, but had significant effects on differentiation with up-shield inoculation having higher differentiation efficiency; the effective embryo ages for in-vitro immature embryo culture ranged from 14 days to 20 days, the optimal embryo age was 16 days; Callus induction had no significant difference among genotypes, but Callus differentiation and regeneration had great difference among genotypes. Differentiation rate presented single-curved trend with the increase of 2,4-D concentration, varying among genotypes, the suitable 2,4-D concentration for most of the cultivars was 1-6 mg/L, 0.2 mg/L NAA 和 0.2 mg/L IAA induced rooting promptly, with rooting rate up to 83.8% and 55.1%, respectively.