详细信息
高效液相色谱法研究当归指纹图谱 被引量:15
Studies on Chromatography Fingerprint(CFP) of Danggui by High-performance Liquid Chromatography
文献类型:期刊文献
中文题名:高效液相色谱法研究当归指纹图谱
英文题名:Studies on Chromatography Fingerprint(CFP) of Danggui by High-performance Liquid Chromatography
作者:赵健雄[1];封士兰[1];胡芳弟[1];刘欣[1];李应东[2]
第一作者:赵健雄
机构:[1]兰州大学;[2]甘肃中医学院
第一机构:兰州大学
年份:2005
卷号:11
期号:4
起止页码:303
中文期刊名:分析测试技术与仪器
外文期刊名:Analysis and Testing Technology and Instruments
基金:甘肃省省长基金资助项目(No.GS015-A45-007;GS024-A43-018)
语种:中文
中文关键词:当归;指纹图谱;高效液相色谱
外文关键词:danggui; chromatographic fingerprint(CFP) ; high-performance liquid chromatography
摘要:目的:研究当归药材的指纹图谱.方法:高效液相色谱法,Hypersil ODS柱(4.6 mm×250 mm,5 μm),Kromasil ODS保护柱(4.6 mm×10mm,5 μm).甲醇-1%醋酸梯度流动相,流速1. 0 mL/min,柱温25℃.以当归对照药材为对照品,色谱峰光谱采集范围:190~400 nm;以阿魏酸为参照物并测定了阿魏酸的含量,检测波长:323nm,流动相:甲醇-1%醋酸(40:60,V/V),流速0.7 mL/min.结果:找出了22个共有峰,其中5号峰为阿魏酸,11个样品与当归对照药材之间的相似度均在90%以上,平均相似度为96.77%.结论:样品处理方法简单,研究所得的当归指纹图谱稳定性、重复性好,可以作为当归极性部分的特征性指纹图谱.
Objective: Study on chromatographic fingerprint of Danggui herbs. Method: High-performance liquid chromatography on a Hypersil ODS column with HOAc(1%)-methanol gradient elution, flow-rate of 1.0 mL/min, column temperature of 25 ℃, standard such as Danggui standard, acquisition wave range was 190-400 nm Determined content of ferulic acid with PAD. Mobile phase: Methanol-1% HOAc (60:40, V/V), flow-rate of 0.7mL/min, detector wave: 323 nm. Result: 22 common peakslwere pointed out, 5th peak was ferulic acid, their similarity of 11 samples were more than 90.0%. Conclusion: Handle method of samples was simple, stable and repeatable. The chromatographic fingerprint of Danggui samples could be used to aqueous solution section of them characterized chromatographic fingerprint.
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