文献类型：期刊文献

中文题名：甘草种子蛋白质提取及SDS-PAGE电泳技术研究

英文题名：Extraction and SDS-PAGE Analysis of Protein from Glycyrrhiza uralensis Fisch.Seed

作者：张延红[1];高素芳[1];陈红刚[1];李金田[1];杜弢[1]

第一作者：张延红

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2016

卷号：35

期号：2

起止页码：21

中文期刊名：种子

外文期刊名：Seed

收录：CSTPCD;;北大核心:【北大核心2014】；

基金："国家基本药物所需重要中药材的原料药材的种子种苗繁育基地"项目(No.国中医药办规财发[2013]41号);甘肃中医药管理局项目"甘草繁殖特性及良种繁育技术研究"(项目编号:GZK-2011-63)

语种：中文

中文关键词：甘草;种子;蛋白质提取;SDS-聚丙烯酰胺凝胶电泳

外文关键词：Glycyrrhiza uralensis Fisch.; seed; extracting method of protein; SDS-PAGE

摘要：目的:建立一套适用于甘草种子蛋白质提取及SDS-PAGE电泳的技术体系,为甘草种子纯度检测提供技术支撑。方法:对甘草种子蛋白质的8种提取方法进行了筛选,在此基础上进一步比较不同的分离胶浓度、不同料液比对甘草种子蛋白质SDS-PAGE电泳的影响,并采用该技术方法对乌拉尔甘草和胀果甘草种子进行鉴定。结果:巯基乙醇法和丙酮沉淀法是适宜的蛋白质提取方法,巯基乙醇法得到12条带,丙酮沉淀法得到9条蛋白质条带,且背景颜色较浅,适宜于进行种子纯度检测。10%分离胶和1∶10的料液比电泳效果较好。乌拉尔甘草和胀果甘草种子有蛋白质条带的差异。结论:建立了一种高质量的甘草种子蛋白质样品的制备方法及SDS-PAGE电泳技术,可用于甘草种子纯度检测。
Object：In order to test the purity of Glycyrrhiza uralensis Fisch.seeds,suitable technology systems of protein extraction and SDS-PAGE were studied.Method：Eight extraction methods for seeds protein extraction were tested.Choosing the best extraction method,the influence of different concentration of separation gels and different solid-liquid ratio on SDS-PAGE was studied.Results：Mercaptoethanol method and acetone precipitation method were suitable for protein extraction method,the method of mercaptoethanol got 12 bands,acetone precipitation got 9protein bands,and the background was distinct,which were suitable for seed purity testing.Electrophoresis effect of 10%separating gel and 1∶10solid-liquid ratio was fine.Seed-protein of Glycyrrhiza uralensis Fisch.and Glycyrrhiza inflate Bat.had differences in protein bands.Conclusion：a high quality of Glycyrrhiza uralensis seed-protein extracting method and SDS-PAGE electrophoresis technology were established,which can be used in seed purity testing.