文献类型：期刊文献

英文题名：Procyanidin B2 Ameliorates Acute Alcoholic Liver Injury by Suppressing the PI3K-Akt/NF-κB Signaling Pathway to Regulate Oxidative Stress and Inflammatory Response

作者：Guo, Jianjin[1];Li, Zhaohuan[2];Gao, Jing[3];Guo, Yan[3];Hou, Yufei[4];Lin, Shaoding[5];Chen, Xia[5];Zhu, Liangdong[5]

第一作者：Guo, Jianjin

通信作者：Lin, SD[1];Chen, X[1];Zhu, LD[1]

机构：[1]Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Nat Prod Chem, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou 730000, Peoples R China;[3]Northwest Univ, Coll Food Sci & Engn, Xian 710069, Shaanxi, Peoples R China;[4]Xi An Jiao Tong Univ, Yulin Hosp, Affiliated Hosp 1, Yulin 719000, Peoples R China;[5]Hunan Univ Med, Affiliated Hosp 1, Huaihua 418000, Hunan, Peoples R China

第一机构：Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Nat Prod Chem, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Hunan Univ Med, Affiliated Hosp 1, Huaihua 418000, Hunan, Peoples R China.

年份：2026

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001668747000001)】；

基金：This work was funded by the Natural Science Foundation of Hunan Province, China (Grant No. 2024JJ9593).

语种：英文

外文关键词：Procyanidin B2; Acute alcoholic liver injury; Network pharmacology; PI3K-Akt/NF-kappa B Pathway; Oxidative stress; Inflammatory response

摘要：Acute alcoholic liver injury is a significant clinical challenge with complex pathogenesis. This study systematically investigated the hepatoprotective effect of procyanidin B2 (PB2) using an integrated approach combining network pharmacology, molecular docking, molecular dynamics simulations, and in vivo/in vitro validation. Network analysis identified 161 common targets and highlighted the PI3K-Akt/NF-kappa B pathway as central. PB2 demonstrated high binding affinity to core targets such as SRC. In a mouse model, PB2 intervention significantly attenuated histopathological damage, reduced oxidative stress markers, improved lipid profiles, and suppressed pro-inflammatory cytokines while enhancing IL-10 expression. Western blot analysis confirmed PB2 dose-dependently inhibited phosphorylation of key proteins in the PI3K-Akt and NF-kappa B pathways. Cellular experiments further validated that PB2 alleviated ethanol-induced injury, apoptosis, and inflammatory response. These findings reveal that PB2 exerts protective effects against acute alcoholic liver injury primarily by modulating the PI3K-Akt/NF-kappa B signaling axis, offering a promising natural therapeutic candidate.