文献类型：期刊文献

英文题名：Genetic Analysis of Reemerging GII.P16-GII.2 Noroviruses in 2016-2017 in China

作者：Ao, Yuanyun[1];Cong, Xin[1];Jin, Miao[1];Sun, Xiaoman[1];Wei, Xiaoman[2];Wang, Jinjin[3];Zhang, Qing[1];Song, Jiao[4];Yu, Jiemei[1];Cui, Jie[2];Qi, Jianxun[5];Tan, Ming[6];Duan, Zhaojun[1]

第一作者：Ao, Yuanyun

通信作者：Duan, ZJ[1]

机构：[1]Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, 155 Changbai Rd, Beijing 102206, Peoples R China;[2]Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China;[3]Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China;[4]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou, Gansu, Peoples R China;[5]Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China;[6]Cincinnati Childrens Hosp Med Ctr, Div Infect Dis, Cincinnati, OH 45229 USA

第一机构：Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, 155 Changbai Rd, Beijing 102206, Peoples R China

通信机构：[1]corresponding author), Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, 155 Changbai Rd, Beijing 102206, Peoples R China.

年份：2018

卷号：218

期号：1

起止页码：133

外文期刊名：JOURNAL OF INFECTIOUS DISEASES

收录：;Scopus(收录号：2-s2.0-85048452701);WOS:【SCI-EXPANDED(收录号:WOS:000434930900016)】；

基金：This study was funded by the Special National Project on Research and Development of Key Biosafety Technologies (grant number 2016YFC1201900) and the National Natural Science Foundation of China (grant numbers 31500139 and 81702007).

语种：英文

外文关键词：norovirus; reemerging GII.P16-GII.2; phylogenetic analysis; histo-blood group antigen; crystal structure

摘要：Background. During 2016-2017, the previously rare GII.P16-GII.2 norovirus suddenly emerged as the predominant genotype causing gastroenteritis outbreaks in China and other countries. Its origin, phylodynamics, and mechanism behind the predominance remain unclear. Methods. Bayesian phylogenetic analyses were performed on 180 full capsid and 150 polymerase sequences of 2016-2017 GII.P16-GII.2 noroviruses in China, and those for all publicly available GII.P16andGII.2 sequences. Saliva-based histo-blood group antigen (HBGA) binding assays and crystal structural analysis were conducted by using the P proteins of 2016-2017 GII.P16-GII.2 noroviruses. Results. The reemerging GII.P16-GII.2 norovirus showed a rapid genetic diversification after its emergence in 2012-2013. The antigenicity and HBGA binding profile of the early 2016-2017 and pre-2016 GII.2 noroviruses were similar. A further variant with a single Val256Ile mutation and the conventionally orientated Asp382 in the VP1 protein showed an expanded HBGA-binding spectrum. Mutations on the surface of polymerase that could alter its function were seen, which may help to accelerate the VP1 gene evolution to 5.5 x 10(-3) substitutions per site per year. This virus can be traced back to Pearl River Delta, China. Conclusions. Our findings provide new insights into GII.2 norovirus epidemics and highlight the necessity of enhanced global surveillance for potential epidemics of rare-genotype noroviruses.