文献类型：期刊文献

中文题名：沙参麦冬汤通过调节EGFR/MEK/ERK信号通路对非小细胞肺癌细胞增殖、凋亡和自噬的影响

英文题名：Impacts of Radix Ophiopogon Decoction on the Proliferation,Apoptosis and Autophagy of Non-small Cell Lung Cancer Cells by Regulating EGFR/MEK/ERK Signaling Pathway

作者：郭晓黎[1];任小宁[1];樊一波[1];庞鹏宇[2];任红艳[3];马静维[4]

第一作者：郭晓黎

机构：[1]宝鸡职业技术学院,陕西宝鸡721013;[2]宝鸡市中医医院消化科;[3]甘肃中医药大学;[4]宝鸡市中医医院呼吸与危重症医学科

第一机构：宝鸡职业技术学院,陕西宝鸡721013

年份：2022

卷号：25

期号：10

起止页码：1707

中文期刊名：中国药师

外文期刊名：China Pharmacist

收录：CSTPCD

语种：中文

中文关键词：沙参麦冬汤;非小细胞肺癌;增殖;凋亡;自噬;表皮生长因子受体/丝裂原活化蛋白激酶激酶/细胞外调节蛋白激酶信号通路;

外文关键词：Radix Ophiopogon decoction;Non-small cell lung cancer;proliferation;Apoptosis;Autophagy;Epidermal growth factor receptor/mitogen-activated protein kinase kinase/extracellular regulated protein kinase signaling pathway;

摘要：目的:探讨沙参麦冬汤(ROD)对非小细胞肺癌(NSCLC)细胞增殖、凋亡和自噬的影响,并分析其潜在机制。方法:制备ROD含药血清,体外培养人NSCLC细胞株A549,MTT法检测不同浓度的ROD含药血清对A549细胞活力的影响,筛选合适的干预浓度;将A549细胞随机分为空白组、阿法替尼组、20%ROD组、20%ROD+EGFR激活剂NSC 228155组;给予相应的干预后,采用细胞集落形成实验检测细胞增殖能力,流式细胞术分析细胞凋亡情况,GFP-LC3转染检测细胞自噬情况,蛋白质印迹(Western blot)检测细胞增殖、凋亡、自噬和表皮生长因子受体(EGFR)/丝裂原活化蛋白激酶激酶(MEK)/细胞外调节蛋白激酶(ERK)通路相关蛋白表达。结果:ROD含药血清可抑制A549细胞增殖,且20%ROD含药血清对A549细胞活力的抑制作用最显著(P<0.05)。20%ROD含药血清可显著减少细胞集落数,升高细胞凋亡率,增加GFP-LC3斑点数,并降低Ki-67、p62蛋白水平和p-EGFR/EGFR、p-MEK/MEK和p-ERK/ERK比值,升高Cleaved Caspase-3/Caspase-3和LC3II/LC3I比值(P<0.05);EGFR抑制剂Afatinib对A549细胞增殖、凋亡、自噬和EGFR/MEK/ERK通路的作用与ROD含药血清相似;使用NSC 228155激活EGFR/MEK/ERK通路可显著减弱ROD含药血清对A549细胞凋亡和自噬的诱导作用以及对细胞增殖的抑制作用(P<0.05)。结论:ROD含药血清可有效抑制A549细胞增殖,并诱导细胞凋亡、自噬,其作用机制可能与抑制EGFR/MEK/ERK通路有关。
Objective:To investigate the impacts of Radix Ophiopogon decoction(ROD)on the proliferation,apoptosis and autophagy of non-small cell lung cancer(NSCLC)cells,and to analyze its potential mechanism.Methods:The ROD-containing serum was prepared,and the human NSCLC cell line A549 was cultured in vitro.The effect of different concentrations of ROD-containing serum on the viability of A549 cells was detected by MTT method,and the appropriate intervention concentration was screened;A549 cells were randomly divided into blank group,afatinib group,20%ROD group,and 20%ROD+EGFR activator NSC 228155 group.After appropriate intervention,the cell proliferation ability was detected by cell colony formation assay,the cell apoptosis was analyzed by flow cytometry,the autophagy was measured by GFP-LC3 transfection,the cell proliferation,apoptosis,autophagy and epidermal growth factor receptor(EGFR)/mitogen-activated protein kinase kinase(MEK)/extracellular regulated protein kinase(ERK)pathway-related protein expression was measured by Western blot.Results:ROD-containing serum was able to inhibit the proliferation of A549 cells,and 20%ROD-containing serum had the most significant inhibitory effect on the viability of A549 cells(P<0.05).20%ROD-containing serum could significantly reduce the number of cell colonies,increase the rate of apoptosis,increase the number of GFP-LC3 puncta,reduce the protein levels of Ki-67 and p62,and the ratios of p-EGFR/EGFR,p-MEK/MEK and p-EGFR-ERK/ERK,and increase the ratios of Cleaved Caspase-3/Caspase-3 and LC3 II/LC3 I(P<0.05);the effects of the EGFR inhibitor afatinib on the proliferation,apoptosis,autophagy and EGFR/MEK/ERK pathway of A549 cells were similar to those of ROD-containing serum;using NSC 228155 to activate the EGFR/MEK/ERK pathway could significantly attenuate the induction of ROD-containing serum on the apoptosis and autophagy of A549 cells and the inhibitory effect on the cell proliferation(P<0.05).Conclusion:ROD-containing serum can effectively inhibit the proliferation of A549 cells,and induce the cell apoptosis and autophagy,and its mechanism may be related to the inhibition of EGFR/MEK/ERK pathway.