文献类型：期刊文献

中文题名：桔梗白散治疗非小细胞肺癌小鼠的作用研究

英文题名：Effects of Platycodon grandiflorum Bai powder in the treatment non-small cell lung cancer rats

作者：袁超[1];李金田[1];梁建庆[2];张毅[1];孔思琪[1];胡蓉[1];张悦[1];刘瑜[1]

第一作者：袁超

机构：[1]甘肃中医药大学中医临床学院,甘肃兰州730000;[2]甘肃中医药大学基础医学院,甘肃兰州730000

第一机构：甘肃中医药大学中医临床学院

年份：2024

卷号：40

期号：11

起止页码：1608

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金资助项目(82160872);甘肃省教育厅“双一流”科研重点基金资助项目(GSSYLXM-05);甘肃省中医药研究中心开放课题基金资助项目(zyzx-2020-zx17);中医学一级学科“岐黄英才”导师专项基金资助项目(2022-13);甘肃中医药大学研究生创新创业基金资助项目。

语种：中文

中文关键词：桔梗白散;顺铂;肺癌;凋亡;B淋巴细胞瘤-2/Bcl-2相关X蛋白;胱天蛋白酶-3

外文关键词：Platycodon grandiflorum Bai powder;cisplatin;lung cancer;apoptosis;B-cell lymphoma-2/Bcl-2 associated X protein;cysteinyl aspartate specific proteinase-3

摘要：目的观察中药复方桔梗白散对Lewis肺癌小鼠皮下移植瘤生长及组织中B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、胱天蛋白酶(caspase)-3、caspase-9表达的影响。方法建立Lewis肺癌移植瘤小鼠模型,将70只SPF级雄性C57BL/6小鼠随机分为空白组、模型组、低剂量实验组、中剂量实验组、高剂量实验组、对照组、联合组。空白组、模型组灌胃0.9%NaCl 0.2 mL;对照组给予等量0.9%NaCl灌胃的同时腹腔注射顺铂25 mg·kg^(-1);高、中、低剂量实验组分别给予193、96、48 mg·kg^(-1)·d^(-1)桔梗白散0.2 mL灌胃;联合组以96 mg·kg^(-1)·d^(-1)桔梗白散0.2 mL灌胃,同时腹腔注射顺铂25 mg·kg^(-1),隔日1次。以流式细胞术检测小鼠骨髓髓源性抑制细胞(MDSCs),以免疫荧光法观察肿瘤细胞中Bcl-2、Bax、caspase-3、caspase-9的表达。结果空白组、模型组、低剂量实验组、中剂量实验组、高剂量实验组、对照组、联合组小鼠骨髓中MDSCs百分比分别为(32.50±2.76)%、(63.13±3.14)%、(48.43±2.23)%、(42.53±1.28)%、(32.93±3.56)%(51.30±4.25)%和(19.90±6.21)%;模型组、低剂量实验组、中剂量实验组、高剂量实验组、对照组、联合组Bax荧光强度分别为10.42±0.68、12.40±1.23、15.14±0.65、22.95±1.76、27.18±1.62和31.61±1.28,Bcl-2荧光强度分别为36.85±0.80、33.92±4.20、28.88±1.01、20.04±2.21、15.69±2.36和6.05±0.73,caspase-3荧光强度分别为5.28±0.44、7.63±0.55、9.66±0.85、14.73±1.18、17.95±1.29和22.92±1.95,caspase-9荧光强度分别为9.48±0.90、11.57±0.72、13.45±0.93、15.73±1.44、19.20±0.96和23.21±1.51。中、高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.05);联合组的上述指标与对照组相比,在统计学上差异均有统计学意义(均P<0.05)。结论桔梗白散能够通过上调Bax、caspase-3、caspase-9表达,下调Bcl-2表达,抑制MDSCs,促进肿瘤细胞凋亡,抑制肿瘤生长。
Objective To observe the effects of traditional Chinese medicine compound Platycodon grandiflorum Bai powder on the growth of subcutaneously implanted tumor and the expression of B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax),cysteinyl aspartate specific proteinase(caspase)-3 and caspase-9 in subcutaneously implanted tumor of Lewis lung cancer mice.Methods The model of transplanted tumor of Lewis lung cancer in mice was established.Seventy SPF male C57BL/6 mice were randomly divided into blank group,model group,low dose experimental group,medium dose experimental group,high dose experimental group,control group and combined group.Blank group and model group were given 0.9%NaCl 0.2 mL by gavage;control group was given0.9%NaCl by gavage and 25 mg·kg^(-1)cisplatin intraperitoneally;high,medium,low dose experimental groups were given 193,96,48 mg·kg^(-1)·d^(-1)Platycodon grandiflorum Bai powder 0.2 mL by gavage,respectively;combined group was given 96 mg·kg^(-1)·d^(-1)Platycodon grandiflorum Bai powder 0.2 mL by gavage,and 25 mg·kg^(-1)cisplatin intraperitoneally,once every other day.The myelogenous suppressor cells(MDSCs)of mouse bone marrow were detected by flow cytometry,and the expressions of Bcl-2,Bax,caspase-3 and caspase-9 in tumor cells were detected by immunofluorescence.Results The percentage of MDSCs in bone marrow of mice in blank group,model group,low dose experimental,medium,high dose experimental group,control group and combination group were(32.50±2.76)%,(63.13±3.14)%,(48.43±2.23)%,(42.53±1.28)%,(32.93±3.56)%,(51.30±4.25)%and(19.90±6.21)%,respectively.The fluorescence intensities of Bax in model group,low dose experimental group,medium dose experimental group,high dose experimental group,control group and combination group were10.42±0.68,12.40±1.23,15.14±0.65,22.95±1.76,27.18±1.62 and 31.61±1.28;Bcl-2 were36.85±0.80,33.92±4.20,28.88±1.01,20.04±2.21,15.69±2.36 and 6.05±0.73;caspase-3 were5.28±0.44,7.63±0.55,9.66±0.85,14.73±1.18,17.95±1.29 and 22.92±1.95;caspase-9 were9.48±0.90,11.57±0.72,13.45±0.93,15.73±1.44,19.20±0.96 and 23.21±1.51.There were significant differences between medium,high dose experimental groups and model group(allP<0.05),and there were significant differences between combined group and control group(allP<0.05).Conclusion Platycodon grandiflorum Bai powder can up-regulating the expression of Bax,caspase-3 and caspase-9,down-regulating the expression of Bcl-2,inhibiting MDSCs,promoting tumor cell apoptosis and inhibiting tumor growth.