文献类型：期刊文献

中文题名：蛇葡萄素钠协同卡铂体外抑制人肺腺癌GLC-82细胞增值的作用

英文题名：The tumor-suppressing effects of ampelopsin sodium combined with carboplatin on human lung adenocarcinoma cell line GLC-82

作者：张艳霞[1,2];缪苗苗[3];吴勇杰[2];李文广[2]

第一作者：张艳霞

机构：[1]甘肃中医学院;[2]兰州大学基础医学院药理学研究所,甘肃省新药临床前研究重点实验室;[3]兰州市第二人民医院

第一机构：甘肃中医药大学

年份：2014

卷号：30

期号：6

起止页码：38

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

语种：中文

中文关键词：蛇葡萄素钠;卡铂;人肺腺癌GLC-82细胞;细胞凋亡;Caspase-3

外文关键词：ampelopsin sodium（蛇葡萄素钠） ; carboplatin; human hmg adenocarcionma cell line GLC-82; Caspase-3

摘要：目的：考察蛇葡萄素钠单用及与卡铂合用对人肺腺癌GLC-82细胞增值的抑制作用,初步探讨蛇葡萄素钠抑瘤作用机制。方法：应用MTT法考察蛇葡萄素钠单用及与卡铂合用对人肺腺癌GLC-82细胞的细胞毒作用;使用流式细胞仪检测凋亡相关基因Caspase-3的表达。结果：MTT实验结果表明,蛇葡萄素钠在浓度为12.5~200μg/ml的剂量范围内对GLC-82细胞的增殖有浓度依赖性的抑制作用;卡铂在浓度为3.13~100μg/ml的剂量范围内对GLC-82细胞的增值有浓度依赖性的抑制作用;25、50μg/ml的蛇葡萄素钠对卡铂25μg/ml抑制GLC-82细胞增殖的作用具有协同效应;流式细胞仪检测结果表明,12.5~50μg/ml的蛇葡萄素钠与卡铂25μg/ml联合用药组作用于GLC-82细胞12 h后,Caspase-3表达上调,且有浓度依赖性。结论：蛇葡萄素钠单用对GLC-82细胞的增殖具有一定的抑制作用,与卡铂合用,对GLC-82细胞增殖具有协同抑制效应;蛇葡萄素钠诱导细胞凋亡的机制之一可能是通过激活细胞内的Caspase-3,从而促进凋亡的发生。
Objective： To investigate the cytotoxic effect and mechanism of ampelopsin sodium（AMP-Na） on human lung adenocarcionma cell line GLC-82 by alone or combined with carboplatin. Methods： Use the MTT colorimetric method to investigate cytotoxicity effect of AMP-Na a- lone or combined with carboplatin to human lung adenocamionma cell line GLC-82; Use flow cytometry （FCM）analysis the express of Caspase-3. Results： Experimental study on cytotoxicity in vitro showed that GLC-82 cells was inhibited by AMP-Na alone in a concentration- dependent manner ranging from 12.5 ~ 200 μg/ml, and was inhibited by carboplatin alone in a concentration-dependent manner ranging from 3.13 ~ 100 μg/ml. Combined with carboplatin 25 μg/ml, AMP-Na 25 and 50 μg/ml had synergistic effect on the proliferation of GLC-82 cells. Flow cytometry analysis showed that 12 hours after AMP-Na （ 12.5 ~50 μg/ml） combined with carboplatin 25 μg/ml to GLC-82 cell, the express of Caspase-3 up-regulation. Conclusion： AMP-Na had depressant effect to the generation of GLC-82 cell, There is a synergistic cytotoxic effect on GLC-82 cells treated with AMP-Na combined with carboplatin. The mechanisms to induce apoptosis by AMP-Na is proba- bly that activation of Caspase-3 mediated signal transduction pathway in cells.