文献类型：期刊文献

中文题名：萎胃宁浓缩丸定性定量方法研究

英文题名：Study on quality and quantity methods of Weiweining concentrated pills

作者：李芸[1];魏舒畅[1];邓沂[1,2];苗小楼[3];刘峰林[1];金辉[1]

第一作者：李芸

机构：[1]甘肃中医学院,兰州730000;[2]安徽中医药高等专科学校,芜湖241000;[3]中国农业科学院兰州畜牧与兽药研究所,兰州730050

第一机构：甘肃中医药大学

年份：2009

卷号：29

期号：12

起止页码：2102

中文期刊名：药物分析杂志

外文期刊名：Chinese Journal of Pharmaceutical Analysis

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：甘肃省科学事业费项目(QS061-C33-26)

语种：中文

中文关键词：萎胃宁浓缩丸;薄层色谱;高效液相色谱;芍药苷

外文关键词：Weiweining pills; TLC; HPLC; paeoniflorin;

摘要：目的:建立萎胃宁浓缩丸定性定量方法。方法:采用TLC法鉴别方中黄芩、黄连、白芍、木香、甘草;采用HPLC法测定芍药苷含量,色谱柱为Phenomenex Luna C18柱(250mm×4.6mm,5μm),流动相为乙腈-0.02%磷酸(15∶85),流速1.0mL·min-1,检测波长230nm;柱温25℃。结果:定性鉴别薄层色谱特征明显;芍药苷浓度在6.14～122.8μg·mL-1范围内线性关系良好(r=0.9997),平均回收率(n=5)为97.7%,RSD=1.7%。结论:该法可以准确地对萎胃宁浓缩丸进行定性、定量,有效地控制该制剂的质量。
Objective：To establish the quality and quantity methods of Weiweining concentrated pills.Methods：Radix Scutellariae,Rhizoma Coptidis,Radix Paeoniae Alba,Radix Aucklandiae,Radix et Rhizoma Glycyrrhizae in this preparation were identified by TLC.The content of paeoniflorin in Weiweining concentrated pills was determined by HPLC.HPLC method was performed on a Phenomenex Luna C18 column（250 mm×4.6 mm,5 μm） with a mobile phase of acetonitrile-0.02% phosphoric acid（15∶85）.The wavelength of detector was set at 230 nm and the column temperature was 25 ℃.Results：Characteristics of identification were very obviously.The linear range of paeoniflorin was 6.14-122.8 μg·mL-1（r=0.9997） and the average recovery was 97.7%（RSD=1.7%）.Conclustion：The methods of qualitative and quantitative analysis are accurate and suitable for effectively controlling quality of Weiweining concentrated pills.