文献类型：期刊文献

中文题名：黄芪甲苷Ⅳ对溃疡性结肠炎大鼠的作用及其机制研究

英文题名：Effect and the underlying mechanism of astragaloside Ⅳ on ulcerative colitis in rats

作者：臧凯宏[1,2];吴建军[1];段海婧[1,2];马清林[1];任远[1,2]

第一作者：臧凯宏

机构：[1]甘肃中医药大学甘肃省中药药理与毒理学重点实验室,兰州730000;[2]甘肃中医药大学药学院,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2019

卷号：35

期号：1

起止页码：48

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金地区基金资助项目(81860728);甘肃省自然科学基金资助项目(17JR5RA162)

语种：中文

中文关键词：黄芪甲苷Ⅳ;溃疡性结肠炎;黏膜通透性;黏膜屏障

外文关键词：astragaloside Ⅳ;ulcerative colitis;mucosal permeability;mucosal barrier

摘要：目的研究黄芪甲苷Ⅳ对溃疡性结肠炎(UC)大鼠的作用及其机制。方法用2,4,6-三硝基苯磺酸钠(TNBS)结肠灌注建立UC大鼠模型。按照体重将大鼠随机分为6组:正常组、模型组、对照组和低、中、高3个剂量实验组,每组10只。正常组与模型组给予0. 5%CMC-Na灌胃;对照组给予柳氮磺胺吡啶300 mg·kg^(-1)灌胃;低、中、高3个剂量实验组分别灌胃黄芪甲苷Ⅳ10,20,40mg·kg^(-1),1次/天,连续8 d。收集结肠并测定组织中髓过氧化物酶(MPO)活性;用酶联免疫吸附法检测结肠肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)水平。结果正常组、模型组和低、中、高3个剂量实验组结肠组织MPO活性分别为(0. 11±0. 04),(4. 66±1. 22),(3. 63±0. 40),(3. 30±0. 68)和(2. 85±0. 57) U·mg^(-1);这5组结肠组织TNF-α含量分别为(176. 92±70. 27),(478. 96±91. 27),(357. 21±96. 22),(328. 37±99. 24)和(287. 09±87. 57) pg·mg^(-1);这5组结肠组织IL-1β水平分别为(402. 59±37. 58),(1045. 83±213. 59),(817. 93±104. 60),(772. 18±146. 94)和(717. 42±82. 44) pg·mg^(-1)。上述这3个指标:模型组与正常组比较,差异均有统计学意义(均P <0. 01); 3个剂量实验组与模型组比较,差异均有统计学意义(均P <0. 05)。结论黄芪甲苷Ⅳ可明显改善UC肠道病变,其作用与减轻炎症反应、提高黏膜屏障功能有关。
Objective To investigate the effect and underlying mechanism of astragaloside Ⅳ on ulcerative colitis(UC)in rats.Methods UC rat model was induced by 2,4,6-three sodium nitrobenzene sulfonate(TNBS)intra-colonic administration.The rats were randomly divided into 6 groups: normal group,model group,control group,as well as low,medium and high dose of experimental groups.Rats in normal group and model group were orally administered with 0.5% CMC-Na,rats in control group were orally treated with salazosulfapyridine(300 mg·kg^-1),while rats in experimental groups were orally administered with astragaloside Ⅳ at the dose of 10,20 and 40 mg.kg^-1,respectively.All the drugs were administered for consecutive 8 days,1 times a day.Colon samples were collected for determining myeloperoxidase(MPO)activity.The level of tumor necrosis factor α(TNF-α)and interleukin-1β(IL-1β)in the colon were determined by enzyme linked immunosorbent assay.Results The MPO activity of colon tissue in normal group,model group,and the three dose of experimental groups were(0.11±0.04),(4.66±1.22),(3.63±0.40),(3.03±0.68),and(2.85±0.57)U·mg^-1,respectively;the colonic TNF-α content in the 5 groups were(176.92±70.27),(478.96±91.27),(357.21±96.22),(328.37±99.24),and(287.09±87.57)pg·mg^-1,respectively;the IL-1β content in the 5 groups were(402.59±37.58),(1045.83±213.59),(817.93±104.60),(772.18±146.94),and(717.42±82.44)pg·mg^-1,respectively.Significant difference was found between normal group and model group(allP<0.01),and significant difference was also found between the three experimental groups and model group(allP<0.05).Conclusion Colonic damage in colitis rats was markedly improved by astragaloside Ⅳ treatment,this effect has close correlation with the decreased colonic inflammation and enhanced mucosa barrier function.