文献类型：期刊文献

中文题名：小秦艽rRNA基因内转录间隔区测序鉴定的初步研究

英文题名：Primary Study on Measuring the InternalTranscribed Spacer l Regions of rRNA Genein seeds of Gentiana dahurica

作者：姬可平[1];张西玲[1];刘丽莎[1];路权云[1];陈彻[1]

第一作者：姬可平

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2003

卷号：28

期号：4

起止页码：313

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：甘肃省自然科学基金项目 (ZS0 11 A2 5 0 58 Y)

语种：中文

中文关键词：小秦艽;中药材;DNA测序;rRNA基因;内转录间隔区

外文关键词：Gentiana dahurica ; nPCR; measure the sequence of DNA;rRNA gene; identification

摘要：目的 :对小秦艽种籽中提取的DNA中rRNA基因内转录间隔区进行碱基序列测定 ,以期从分子水平建立对秦艽种籽的鉴定标准。方法 :常规提取小秦艽种籽DNA ,利用合成的特异性引物对其DNA中rRNA基因内转录间隔区进行套式PCR扩增 ,将扩增产物以四色荧光标记的双脱氧末端终止循环法进行碱基序列测定。结果 :经琼脂糖凝胶电泳证实rRNA基因内转录间隔区PCR扩增产物存在 ,测序后得到了小秦艽种籽rRNA基因内转录间隔区的碱基序列。结论
Objective: To amplify the PCR with the internal transcribed spacerl regions measure the base sequence of the amplified products of DNA, and to set up an identified standard on the level of molecule. Method: DNA from the seeds of G. dahurica was extracted by conventional method, and composed peculiar primer was used to amplify with the internal transcribed spacerl regions of the rRNA gene, and the base sequence of the amplified products by stopping the circle of the end of double deoxidation of four color fluorescent mark was measured . Result: It was proved by agar sugar gel electrophoresis that the PCR amplified products of the internal transcribed spacerl regions of the rRNA gene existed. The base sequence of the seeds of G. dahurica 's internal transcribed spacerl regions of the rRNA gene was measured. Conclusion: To measure the base sequence of internal transcribed spacerl regions of the rRNA gene in the seeds of G. dahurica 's is a method to identify vegetal Chinese traditional medicine on the level of molecule.[