文献类型：期刊文献

中文题名：当归与激素合用对哮喘小鼠的治疗作用及其机制

英文题名：Effects of combined administration of Radix Angelicae Sinensis and hydrocortisone on the therapeutic action in the murine asthma model

作者：王志旺[1,2];付晓艳[1];姚楠[1];巩子汉[1];程小丽[1];段海婧[1];任远[1,2]

第一作者：王志旺

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃省中药药理与毒理学重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：34

期号：4

起止页码：379

中文期刊名：中国应用生理学杂志

外文期刊名：Chinese Journal of Applied Physiology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；PubMed;

基金：国家自然科学基金(81460668);甘肃省自然科学基金(1606RJZA011;1310RJZA086);甘肃省中药药理与毒理学重点实验室项目基金(ZDSYS-KJ-2016-004)

语种：中文

中文关键词：当归;氢化可的松;小鼠;哮喘;高迁移率族蛋白B1;Toll样受体4;核因子κB;血液流变学

外文关键词：Radix Angelicae Sinensis （RASI）;hydrocortisone;mouse;asthma;high-mobility group box 1（HMGB1）;Toll-like receptors 4 （TLR4）;nuclear factor-κB （NF-κB）;hemorheology

摘要：目的:探讨当归与激素合用对哮喘小鼠的治疗作用及其机制。方法:取BALB/c小鼠随机分为对照组、血瘀模型组、哮喘模型组、氢化可的松组、当归组、氢化可的松与当归合用组(n=12)。采用卵白蛋白致敏、激发,复制哮喘模型;使用氢化可的松复制血瘀模型。观测当归、氢化可的松及其合用对小鼠血液流变学(全血黏度与血清TXB2含量)和哮喘(哮喘行为学、肺功能、肺指数与肺组织含水量)的影响,采用ELISA法、免疫组化法测定相关细胞因子和HMGB1/TLR4/NF-κB蛋白质的表达水平。结果:8 g/kg当归、0.05 g/kg氢化可的松及其合用可明显缓解哮喘行为学指标,提高肺功能,降低肺指数及肺组织含水量,降低BALF中TNF-α、IL-1β及IL-6水平,抑制肺组织HMGB1、TLR4及NF-κB蛋白质的高表达;当归与氢化可的松合用对提高哮喘小鼠肺功能、降低相关细胞因子(TNF-α、IL-1β及IL-6)水平明显优于单独使用当归组,抑制肺组织中TLR4和NF-κB蛋白质表达的作用优于单独使用当归或氢化可的松。二者合用后可缓解氢化可的松所致的小鼠血清TXB2含量升高、血液粘稠度增加。结论:当归与激素及其合用具有明显的平喘作用,抑制HMGB1/TLR4/NF-κB蛋白质合成是其平喘的机制之一。当归与激素合用改善肺功能的作用更强,可能与加强抑制TLR4/NF-κB合成有关。合用当归可缓解激素在治疗哮喘时引起的血液流变学异常变化。
Objective： To investigate the effects of Radix Angelicae Sinensis（ RASI） and hydrocortisone combination on the murine asthma model and the mechanism. Methods： BALB/c mice were randomly divided into control group,blood stasis model group,asthma model group,HSS group,RASI group and RASI＋HSS group（ n = 12）. Ovalbumin（ OVA） was used to replicate mice asthma model and hydrocortisone sodium succinate（ HSS） to copy blood stasis model. Effects of RASI,HSS and their combination on hemorheology,anti-asthma（ asthmatic behaviors,lung function,lung index and water content in lung tissue） were observed. and anti-asthma mechanisms The expression of relative cytokines,high-mobility group box 1（ HMGB1）,toll-like receptor 4（ TLR4） and nuclear factor-κB（ NF-κB） was detected by ELISA and immunohistochemistry respectively. Results： Eight g/kg RASI,0.05 g/kg HSS and their combination could significantly relieve asthma behavioral indicators,improve lung function,reduce lung index and water content in lung tissue,decrease the levels of TNF-α,IL-1β and IL-6 in broncho-alveolar lavage fluid（ BALF）,and inhibit the high expression of HMGB1,TLR4 and NF-κB in lung tissue. The improvement of lung function and the decrease in level of relative cytokines（ TNF-α、IL-1β 及 IL-6） were better in RASI＋HSS group than those in RASI group and HSS group,and the inhibition of protein expression of TLR4 and NF-κB was also too. Combined administration of RASI and hydrocortisone could decrease serum thromboxane B2（ TXB2）content and blood viscosity,which were increased induced by hydrocortisone. Conclusion： Combined administration of RASI and hydrocortisone have obvious anti-asthma effects and one of the mechanisms is to inhibit protein synthetization of HMGB1,TLR4 and NF-κB.The combined administration of RASI and hydrocortisone has stronger improvement of lung function than that of RASI and hydrocortisone alone,and it may be related to the inhibition of TLR4 and NF-κB synthetization. The combined administration of RASI can alleviate abnormal changes of hemorheology induced by hydrocortisone in treatment of asthma.