文献类型：期刊文献

英文题名：PGK1: A Common Biomarker and Therapeutic Target Linking Sarcopenia and Osteoporosis Through Fibroblast-Mediated Pathways

作者：Zhang, Kun[1];Li, Hailong[2,3,4,5];Chen, Xinhong[2];Tang, Ping[2];Wang, Meng[4];Yang, Chunting[4];Su, Rong[4];Gao, Xiaqin[4];Zhang, Fan[3];Han, Juan[1,6]

第一作者：Zhang, Kun

通信作者：Han, J[1];Li, HL[2];Li, HL[3];Li, HL[4];Li, HL[5];Han, J[6]

机构：[1]Gansu Prov Tradit Chinese Med Hosp, Dept Trauma Orthoped, Lanzhou, Peoples R China;[2]Shantou Univ, Luohu Clin Coll, Sch Med, Dept Gen Practice, Shenzhen, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Affiliated Hosp, Dept Geriatr, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Sch Clin Med 1, Dept Internal Med, Lanzhou, Peoples R China;[5]Shenzhen Integrated Tradit Chinese & Western Med H, Dept Geriatr, Shenzhen, Peoples R China;[6]Gansu Prov Tradit Chinese Med Hosp, Dept Pediat, Lanzhou, Peoples R China

第一机构：Gansu Prov Tradit Chinese Med Hosp, Dept Trauma Orthoped, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Tradit Chinese Med Hosp, Dept Trauma Orthoped, Lanzhou, Peoples R China;[2]corresponding author), Shantou Univ, Luohu Clin Coll, Sch Med, Dept Gen Practice, Shenzhen, Peoples R China;[3]corresponding author), Gansu Univ Tradit Chinese Med, Affiliated Hosp, Dept Geriatr, Lanzhou, Peoples R China;[4]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Dept Internal Med, Lanzhou, Peoples R China;[5]corresponding author), Shenzhen Integrated Tradit Chinese & Western Med H, Dept Geriatr, Shenzhen, Peoples R China;[6]corresponding author), Gansu Prov Tradit Chinese Med Hosp, Dept Pediat, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;[10735b845793de6ae2b30]甘肃中医药大学第二附属医院;

年份：2025

卷号：19

期号：1

外文期刊名：IET SYSTEMS BIOLOGY

收录：;EI(收录号：20254219338407);Scopus(收录号：2-s2.0-105018647323);WOS:【SCI-EXPANDED(收录号:WOS:001648567700007)】；

基金：This work was supported by Entrepreneurship Project of Science and Technology Bureau of Chengguan District of Lanzhou city (#Chengke [2023] No. 38, 2023-2-5). Shenzhen Key Medical Discipline Construction Fund (No. SZXK062).

语种：英文

外文关键词：big data; bioinformatics; data analysis; molecular dynamics method; network analysis; signal transduction

摘要：Sarcopenia and osteoporosis share pathophysiological links, but their co-occurrence mechanisms remain unclear. This study aimed to identify molecular mediators of their co-development using bioinformatics. Datasets for sarcopenia (GSE56815) and osteoporosis (GSE9103) were retrieved from GEO. Differentially expressed genes (DEGs) were analysed via edgeR and limma. Gene ontology (GO), Kyoto encyclopaedia of genes and genomes (KEGG) and weighted gene co-expression network analysis (WGCNA) identified shared pathways and hub genes. Protein-protein interaction (PPI) networks were constructed using STRING and Cytoscape. We validated hub genes in independent datasets (GSE13850, GSE8479) and assessed via ROC curves. Immune infiltration, single-cell analysis and drug prediction were performed. We identified 134 common DEGs (30 upregulated, 104 downregulated). WGCNA and PPI analysis revealed 14 hub genes (APOE, CDK2, PGK1, HRAS, RUNX2 etc.), all with ROC-AUC > 0.6. PGK1 was consistently downregulated in both diseases and linked to 21 miRNAs and six transcription factors (HSF1, TP53, JUN etc.). Single-cell analysis localised PGK1 predominantly in skeletal muscle fibroblasts. DrugBank identified lamivudine as a potential PGK1-targeting therapeutic. PGK1 emerged as a central downregulated gene in sarcopenia and osteoporosis, enriched in fibroblasts and modulated by lamivudine. These findings highlight PGK1 as a shared diagnostic and therapeutic target, offering insights into musculoskeletal crosstalk.