文献类型：期刊文献

中文题名：酸碱处理法纯化白花泡桐叶中熊果酸的工艺研究

英文题名：Experimental Study on Purifying Ursolic Acid in Leaves of Paulownia fortunei(Seem.) Hemsl.by Acid-alkali Method

作者：李阳[1,2];陈旅翼[3];余晓晖[1,2];赵磊[1,2]

第一作者：李阳

机构：[1]甘肃中医学院,甘肃兰州730000;[2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000;[3]中南民族大学,湖北武汉430074

第一机构：甘肃中医药大学

年份：2013

卷号：41

期号：8

起止页码：3371

中文期刊名：安徽农业科学

外文期刊名：Journal of Anhui Agricultural Sciences

语种：中文

中文关键词：酸碱处理;白花泡桐叶;熊果酸;纯化

外文关键词：Acid-alkali method;Leaves of Paulownia fortunei(Seem.) Hemsl;Ursolic acid;Purification

摘要：[目的]研究通过酸碱处理的方法初步纯化白花泡桐叶提取液中熊果酸的工艺。[方法]采用HPLC测定熊果酸含量,利用单因素试验及正交试验考察碱化pH值、酸化pH值、稀释倍数对熊果酸转移率和纯度的影响。[结果]白花泡桐叶中熊果酸的初步纯化最佳工艺为:碱化pH=10、酸化pH=4、稀释倍数为1.5倍;在此工艺条件下,转移率达到了90.23%,纯度也达到了26.48%。[结论]该工艺简单易行,为泡桐叶中熊果酸的纯化找到了一条适合大工业生产的路线。
[Objective] The study aimed to explore the technique of purifying urasolic acid in the extract of Paulownia fortunei leaves by acid-alkali method.[Method] HPLC was adopted to measure the content of ursolic.The effect of pH value of basification and acidification,dilution factor on the transfer ratio and purity of ursolic acid were tested by the single factor exploration and orthogonal design.[Result] The optimum condition to purify ursolic acid in P.fortunei leaves is under the pH 10 of basification and pH 4 of acidification and 1.5 of dilution factor,the transfer rate and purity are up to 90.23%,26.48%,respectively.[Conclusion] Purifying by this technique is simple and feasible,which enables mass industrial production to purify ursolic acid in P.fortunei leaves.