文献类型：期刊文献

中文题名：当归黄芪醇提物对糖尿病胃轻瘫模型大鼠的治疗作用

英文题名：Therapeutic Effects of Ethanol Extracts of Angelica sinensis and Astragalus mongholicus in Diabetic Gastroparesis Rats

作者：景琪[1];吴国泰[1,2];杜丽东[1,2];刘五州[1];李应东[1];任远[1,2]

第一作者：景琪

机构：[1]甘肃中医学院,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2014

卷号：37

期号：8

起止页码：1415

中文期刊名：中药材

外文期刊名：Journal of Chinese Medicinal Materials

收录：MEDLINE(收录号：25726651);CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金："十二五"国家科技支撑计划项目子课题(2011BAI05B0206);甘肃省自然科学基金项目(1212RJZA079);甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2012-004)

语种：中文

中文关键词：当归黄芪醇提物;糖尿病胃轻瘫;胃动素;胃泌素;Cajal间质细胞

外文关键词：The ethanol extract of Angelica sinensis and Astragalus mongholicus ; Diabetic gastroparesis ; Motilin ; Gastrin; ICC

摘要：目的:研究当归黄芪(1∶2)醇提物对糖尿病胃轻瘫(DG)模型大鼠的治疗作用。方法:SD雄性大鼠腹腔注射四氧嘧啶(200 mg/kg)建立糖尿病大鼠模型,阳性对照组大鼠灌胃二甲双胍和西沙比利混合溶液(含盐酸二甲双胍175 mg/kg和西沙比利3.5 mg/kg),当归黄芪醇提物高、低剂量组大鼠分别灌胃当归黄芪醇提物(生药10.5、5.2 g/kg),1次/d,共42 d。观察各组大鼠的状态,每隔14 d称体质量,并测空腹血糖值(FBG)和24 h进食量及饮水量,末次给药后1 h测定大鼠胃肠推进指数,股动脉取血,制备血浆和血清,ELISA试剂盒测定血浆胃动素(MOT)及血清胰高血糖素(GLG)和胃泌素(GAS)水平,处死大鼠,取胃窦组织,在光镜和电镜下观察平滑肌细胞和ICC细胞的组织形态。结果:给药28 d,当归黄芪醇提物高剂量能显著增加模型大鼠体质量、24 h进食量及减少24 h饮水量(P<0.05);给药42 d,当归黄芪醇提物高、低剂量均能增加模型大鼠的体质量(P<0.05或P<0.01),高剂量能增加模型大鼠24 h进食量、减少24 h饮水量、降低FBG(P<0.05);给药42 d,当归黄芪醇提物高剂量能显著增加模型大鼠胃肠推进指数(P<0.05),当归黄芪醇提物高、低剂量能显著降低大鼠血浆MOT、血清GAS含量(P<0.05或P<0.01),高剂量组大鼠血清GLG亦显著降低(P<0.01);当归黄芪醇提物高、低剂量组大鼠胃黏膜及黏膜下层充血减轻,胃窦平滑肌细胞及腺体细胞排列较整齐,细胞间隙稍大,平滑肌纤维化病变改善。结论:当归黄芪醇提物对糖尿病胃轻瘫模型大鼠具有一定的治疗作用,作用机理与降低FBG、MOT和GAS水平有关。
Objective：To observe therapeutic effects of the ethanol extract of Angelica sinensis and Astragalus mongholicus（1∶ 2）in diabetic gastroparesis（DG）rats. Methods：Diabetic rats were induced by intraperitoneal injection of alloxan（200 mg /kg）. DG was based on gastrointestinal motility index and the character of stool in diabetic rats. The metformin and cisapride mixed solution（containing metformin 175 mg /kg and cisapride 3. 5 mg /kg）was intragastric administrated in the positive control group,the ethanol extract of Angelica sinensis and Astragalus mongholicus was intragastric treated in the high dose（10. 5 g /kg）and low dose（5. 2 g /kg）group rats,1 /d for 42 consecutive days. The body weight,fasting blood glucose（FBG）value and 24 h intake and drinking amount were detected at interval of14 days. The gastrointestinal propulsion index,motilin（ MOT）,glucagon（ GLG） and gastrin（ GAS） contents in the blood were detected after the last administration. And then the gastric antrum smooth muscle cells and interstitial cells of Cajal were observed in microscope. Results：The general state and stool had been improved,and the body weight and 24 h intake were significantly increased,and 24 h drinking in the high and low dose groups at 28 d of administration were reduced. At the 42 th day,the body weight and 24 h intake were increased,24 h drinking water and FBG were reduced,and the MOT,GAS and GLG contents were decreased in the high dose group. Gastric mucosa and gastric smooth muscle tissue morphology were significantly improved. Conclusion：The therapeutic effect of ethanol extracts of Angelica sinensis and Astragalus mongholicus was obvious in DG rats. Its mechanism of action was associated with FBG,MOT and GAS levels decreasing.