文献类型：期刊文献

中文题名：辐射诱发大鼠体内旁效应中肺及肾组织凋亡相关基因mRNA的表达变化

英文题名：Expression of apoptosis-related genes in lung and kidney tissues of rats with radiation-induced bystander effects

作者：张朝宁[1,2];李金田[1,2];刘永琦[2];蔺兴遥[2]

第一作者：张朝宁

机构：[1]甘肃中医药大学中医临床基础教研室,甘肃兰州730000;[2]敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学中医临床学院

年份：2018

卷号：30

期号：1

起止页码：42

中文期刊名：癌变．畸变．突变

外文期刊名：Carcinogenesis,Teratogenesis & Mutagenesis

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金项目(81460695)

语种：中文

中文关键词：辐射旁效应;凋亡相关基因;肺肾组织;mRNA表达

外文关键词：radiation induced bystander effect;apoptosis-related genes;lung and kidney tissue;mRNA expression

摘要：目的:探讨不同剂量X射线辐射诱发大鼠体内旁效应中肺和肾组织凋亡相关基因的表达变化。方法:70只雄性Wistar大鼠随机分为正常对照组和不同剂量、不同时间的辐射组,共10组,每组7只。除正常对照组外,其余各组大鼠分别给予2、4、8 Gy X射线单次照射右肺部,铅皮屏蔽身体其余部位。分别于照射后6、24、48 h处死辐射各组大鼠,实时荧光定量PCR检测大鼠右肺、左肺、左肾组织中Cytc、Caspase-9、Caspase-3、Bax和Bcl-2 mRNA表达的变化。结果:与正常对照组比较,2、4、8 Gy辐射各组大鼠右肺、左肺、左肾中Cytc、Caspase-9、Caspase-3 mRNA表达量在照射后均明显升高(P<0.05或P<0.01)。除右肺8 Gy辐射后48 h组和左肾2 Gy辐射后6 h组之外,其余辐射组Bax mRNA表达量均明显升高,与正常对照组比较,差异均有统计学意义(P<0.05或P<0.01)。除左肾8 Gy辐射后6 h组之外,其余辐射组Bcl-2 mRNA表达量均降低,与正常对照组比较,差异均有统计学意义(P<0.05或P<0.01)。结论:不同剂量X射线照射大鼠右肺部后,右肺组织中Cytc、Caspase-9、Caspase-3、Bax表达均显著上调,Bcl-2明显下调。左肺和左肾中未直接受照射组织也对X射线辐射产生了响应,辐射诱导了体内旁效应的产生。各辐射剂量之间、各时间点之间尚未发现明显的效应差异。
OBJECTIVE： To investigate expression of apoptosis-related genes during different dose of X-ray-induced bystander effects in rats. METHODS：70 male Wistar rats were randomly divided into the normal control group and the different irradiation groups. For the latter groups,the right lungs of the rats were radiated with 2,4,8 Gy X-rays. During irradiation,the rest of the body was covered with lead shielding. At 6,24 and 48 h respectively after radiation,7 rats of each radiation group were executed. Variations in mRNA expression of Cytochrome C,Caspase-9,Caspase-3,Bax and Bcl-2 in the right lung,left lung and left kidney tissue were detected with quantitative polymerase chain reaction （qPCR）. RESULTS：The mRNA expressions of Cytochrome C,Caspase-9 and Caspase-3 in the right lung,left lung and left kidney tissue of the irradiated groups were up-regulated at all time points. However,the mRNA expressions of Bcl-2 were down-regulated at all time points in the right and left lungs. The observed changes were significantly different in comparison with the normal control group （P〈0.05 or P〈0.01）. In the right lung,expressions of Bax mRNA were significantly increased in all irradiated groups except the 8 Gy dose group at 48 h （P〈0.05 or P〈0.01）. In the left lung,expressions of Bax mRNA were significantly increased in each radiation group （P〈0.01）. In the left kidney,expressions of Bax mRNA were significantly increased in all irradiated groups except the 2 Gy dose group at 6 h. Compared with the normal control group,the differences were statistically significant （P〈0.01）. Expressions of Bcl-2 mRNA were decreased at all time points after irradiation except the 8 Gy dose group at 6 h and the difference was statistically significant comparedwith the normal control group （P〈0.05 or P〈0.01）. CONCLUSION：After the right lungs of the rats were irradiated by different doses of X-rays,expressions of Cytochrome C,Caspase-9,Caspase-3 and Bax in the right lung tissue were up-regulated. While expressions of Bcl-2 were down-regulated. The left lung and left kidney tissues that were not irradiated also responded to the X-rays. And the radiation-induced bystander effects were observed in vivo. Between every radiation dose and every time point,there were no apparently different effects.