文献类型：期刊文献

中文题名：西黄丸对大鼠下丘脑-垂体-卵巢轴中各激素与卵巢受体水平的影响

英文题名：Effects of Xihuang Pill on levels of hormones and ovarian receptors in hypothalamic-pituitary-ovarian axis in rats

作者：赵敏[1];连小龙[1];刘景楠[1];苏杉[1];兰咏梅[2];韩涛[1]

第一作者：赵敏

机构：[1]甘肃中医药大学药学院,兰州730000;[2]西北民族大学医学院,兰州730030

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2018

卷号：34

期号：20

起止页码：2417

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区基金资助项目(81560722)

语种：中文

中文关键词：西黄丸;下丘脑-垂体-卵巢轴;激素;受体;脏器指数

外文关键词：Xihuang Pill;hypothalamic - pituitary - ovarian axis;hormones;receptor;organ index

摘要：目的研究西黄丸对大鼠下丘脑-垂体-卵巢轴中各激素、受体水平及卵巢、子宫脏器指数的影响。方法取Wistar雌性未孕大鼠,按随机数表法将大鼠分为5组:空白组、对照组和高、中、低3个剂量实验组,每组10只。对照组给予1. 8 mg·kg^(-1)·d^(-1)他莫昔芬灌胃;高、中、低3个剂量实验组分别给予2. 16,1. 08,0. 54 g·kg^(-1)·d^(-1)西黄丸灌胃,空白组灌胃等体积蒸馏水,连续31 d。以酶联免疫吸附法检测血清中促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、促黄体生成素(LH)、雌二醇(E2)、孕激素水平;用免疫印迹技术检测卵巢中卵泡刺激素受体(FSHR)和促黄体生成素受体(LHR)表达量。结果空白组、对照组和高、中、低3个剂量实验组的GnRH分别为(14. 99±2. 33),(14. 63±3. 39),(11. 90±1. 11),(5. 55±1. 05),(14. 82±1. 47) ng·L^(-1);高、中2个剂量实验组与空白组比较,GnRH水平显著降低,差异均有统计学意义(均P <0. 01);这5组的E2分别为(16. 84±4. 20),(9. 10±4. 76),(13. 38±2. 12),(13. 45±4. 14),(16. 32±4. 23) ng·L^(-1),对照组与空白组比较,E_2水平显著降低,差异有统计学意义(P <0. 01)。这5组的孕激素分别为(164. 46±14. 90),(189. 99±23. 35),(187. 12±17. 23),(196. 25±15. 87),(173. 26±14. 07)pmol·L^(-1);对照组和高、中2个剂量实验组与空白组比较,孕激素水平显著升高,差异均有统计学意义(P <0. 05或P <0. 01)。结论西黄丸能降低大鼠血清中GnRH和E2的含量而增加孕激素的含量,说明西黄丸能够对下丘脑-垂体-卵巢轴相关激素产生影响。
Objective To explore the influence of Xihuang Pill on the levels of hormones and receptors in hypothalamus -pituitary- ovary axis and the index of ovary and uterus organs in rats. Methods Wistar female non-pregnant rats were randomly divided into5 groups （n = 10 in each group） ：blank group,control group and medium,high and low dose experimental groups. Each rat in the control group was given 1.8 mg - kg^-1· d^-1 tamoxifen ;and each rat in the high, middle and low dose experimental groups was given 2. 16,1.08 and 0. 54 g·kg^-1 · d^-1 Xihuang Pill for gastric perfusion, and the blank group was given distilled water with equal volume. The serum levels of gonadotropin - releasing hormone （ GnRH ）, follicle stimulating hormone （ FSH ） and luteinzing hormone （ LH ）, estra- diol（ E2 ）, progesterone were detected by enzyme linked immunosorbent assay. The expression of follicle stimulating hormone receptor （FSHR） and luteinizing hormone receptor （LHR） in ovaries were detected by Western blot. Results GnRH in blank group, control group and high, middle and low dose ex- perimentalgroups were （14.99±2.33）,（14.63 ±3.39）,（11.90± 1.11）, （5.55 ± 1.05）,（14.82 ±1.47） ng· L^-1, respectively;comparison between high, middle dose experimental groups and blank group, the difference was significant（ all P 〈 0.01） .E2 in the 5 groups were （16.84± 4.20）, （9.10 ± 4. 76）, （13.38 ±2. 12）, （13.45±4. 14）, （16. 32±4. 23） ng · L^-1, respectively; comparison between control group and blank group, the difference was significant （ P 〈 0. 01 ）. Progesterone in the 5 groups were （ 164. 46 ± 14.90 ）, （ 189. 99 - 23.35 ） , （ 187.12± 17.23）, （ 196.25±15.87）, （ 173.26 ±14. 07） pmol·L^-1 ,respectively;comparison between control group and high,middle dose experimental groups with blank group,the difference was significant（P 〈 0. 05 or P 〈0. 01 ）. Conehmion Xihuang Pill can reduce the contents of GnRH and E2 in serum of rats and increase the content of progesterone, which indicates that Xihuang Pill can have an influence on hypothalamic -pituitary -ovarian axis related hormones.