文献类型：期刊文献

中文题名：中药汤剂治疗女性围绝经期综合征的用药规律及机制探究

英文题名：Study on Medication Rule and Mechanism of Chinese Herbal Decoction in the Treatment of Female Perimenopausal Syndrome

作者：卫若楠[1];张伟[1,2];周谷城[1];康梦娇[1];周丹妮[1];陈佳[1];赵林华[2]

第一作者：卫若楠

机构：[1]甘肃中医药大学,兰州730000;[2]中国中医科学院广安门医院,北京100053

第一机构：甘肃中医药大学

年份：2023

卷号：18

期号：8

起止页码：1160

中文期刊名：世界中医药

外文期刊名：World Chinese Medicine

收录：CSTPCD

基金：国家中医药管理局“中医药传承与创新‘百千万’人才工程(岐黄工程)岐黄学者”项目(国中医药人教函[2018]284号)。

语种：中文

中文关键词：围绝经期综合征;中药汤剂;数据挖掘;网络药理学;分子对接;用药规律;作用机制

外文关键词：Perimenopausal syndrome;Chinese herbal decoction;Data mining;Network pharmacology;Molecular docking;Medication rule;Mechanism

摘要：目的:基于数据挖掘、网络药理学及分子对接技术探究中药汤剂治疗女性围绝经期综合征(MPS)的用药规律及核心组方治疗MPS的作用机制。方法:在国家知识基础设施数据库(CNKI)、中国生物医学文献数据库(CBM)、中国学术期刊数据库(CSPD)及中文科技期刊数据库(CCD)搜集众医家治疗MPS的基础方剂,采用中医传承计算平台(V3.0)分析用药规律,挖掘核心组方。运用网络药理学探究核心组方治疗MPS的作用机制,再利用分子对接技术对其结果进行验证。结果:本研究收集方剂704首,涉及中药235味,对所涉方剂进行药物频次、性味归经等分析后筛选出核心组方(山药-山茱萸-熟地黄-柴胡-白芍-当归)。运用网络药理学筛选核心组方共72个活性成分,97个核心靶点。京都基因和基因组百科全书(KEGG)通路富集分析显示核心组方治疗女性MPS可能与促乳素信号通路、磷脂酰肌醇-3激酶/蛋白激酶B(PI3K-AKT)信号通路等有关。分子对接显示,雌激素受体Alpha基因(ESR1)与羟基芫花素等有较好的结合力。结论:中药汤剂治疗MPS以滋阴疏肝为主要治则,核心组方的挖掘为中医药治疗MPS标准化、国际化提供些许思路。网络药理学及分子对接结果为核心组方治疗MPS提供了理论依据。
Objective:According to data mining,network pharmacology,and molecular docking technology,the medication rule of Chinese herbal decoction and the mechanism of core formulas in the treatment of female perimenopausal syndrome(MPS)were explored.Methods:The basic prescriptions of many physicians for the treatment of MPS were collected from four Chinese databases:China national knowledge infrastructure(CNKI),Chinese biomedical literature database(CBM),China Science Periodical Database(CSPD),and Chinese citation database(CCD).The traditional Chinese medicine inheritance computing platform(V3.0)was used to analyze their medication rules and mine core formulas.Network pharmacology was used to explore the mechanism of the core formula in the treatment of MPS,and the results were verified by molecular docking technology.Results:In this study,a total of 704 prescriptions were collected,involving 235 herbs,and the involved prescriptions were analyzed in terms of drug frequency,properties,tastes,and meridians.Then the core formula consisting of Rhizoma Dioscoreae,Cornus Officinalis Sieb.Et Zucc.,Rehmanniae Radix Praeparata,Radix Bupleuri,Paeoniae Radix Alba,and Angelicae Sinensis Radix was selected.A total of 72 active components and 97 core targets of the core formula were selected by using network pharmacology.Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis showed that the treatment of female MPS by core formulas may be related to the prolactin signal pathway and phosphatidylinositol-3 kinase/protein kinase B(PI3K-AKT)signal pathway,etc.Molecular docking showed that the estrogen receptor alpha gene(ESR1)could well bind to Hydroxygenkwanin.Conclusion:The main therapeutic principle of Chinese herbal decoction in treating MPS is nourishing yin and soothing the liver.The mining of the core formulas provides some ideas for the standardization and internationalization of traditional Chinese medicine in the treatment of MPS.The results of network pharmacology and molecular docking provide a theoretical basis for the treatment of MPS by core formulas.