文献类型：期刊文献

英文题名：Fast separation and purification of oleuropein from olive (Olea europaea L.) leaves using amine-functionalized polymer microspheres

作者：Li, Xiaodong[1,2];Zhang, Le[1,2];Fan, Yuhang[1,2];Wang, Xiaofang[3];Pei, Dong[2];Di, Duolong[1,2];Liu, Baoqian[2]

第一作者：Li, Xiaodong

通信作者：Di, DL[1];Pei, D[2];Di, DL[2];Liu, BQ[2]

机构：[1]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China;[3]Gansu Pharmaceut Grp Technol Innovat Res Inst Co L, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2026

卷号：229

外文期刊名：REACTIVE & FUNCTIONAL POLYMERS

收录：;EI(收录号：20264021585169);Scopus(收录号：2-s2.0-105052027834);WOS:【SCI-EXPANDED(收录号:WOS:001896843300001)】；

基金：This work was financially supported by the Gansu Science Fund for Basic Creative Research Groups (25JRRA469) ; the Sciences Foundation of Shandong Province (ZR2024QB168) ; Key Cultivation Projects during the "15th Five-Year Plan" Period of LICP (KCP155C09) ; the Gansu Science and Technology Major Project (26ZDLA002, 24ZD17FA003) ; the Longnan Science and Technology Plan Project (2025ZD08) .

语种：英文

外文关键词：Oleuropein; Separation and purification; Amination; Polymer microspheres

摘要：Macroporous adsorption resins are widely used for the separation and purification of natural products, however, commercial resins often exhibit limited selectivity, modest separation efficiency, and long processing times. To address these limitations, we synthesized PM-N by grafting 2-chloroethylamine onto the surface of PS-DVB microspheres, thereby reducing surface hydrophobicity and introducing amine functional groups to enhance selectivity toward oleuropein from olive leaves. Characterization confirmed successful modification without altering the microsphere morphology or pore structure. Adsorption experiments showed that the adsorption isotherms were well fitted by the Langmuir model, while the adsorption kinetics followed a pseudo-second-order model, with equilibrium reached within 10 min. Adsorption capacity increased with temperature, and thermodynamic analysis indicated a spontaneous and endothermic adsorption process. PM-N exhibited a maximum adsorption capacity of 127.01 mg & sdot;g- 1. Under optimized static adsorption conditions, oleuropein purity increased from 19.01% to 77.00%, corresponding to a 4.05-fold enrichment. Competitive adsorption experiments confirmed the selectivity of PM-N, and XPS analysis suggested that adsorption was driven by hydrophilic interactions, hydrogen bonding, ion exchange, and it-it interactions. These results provide a rapid, efficient, and selective strategy for oleuropein enrichment and offer a useful reference for the purification of natural products.