文献类型：期刊文献

中文题名：芍药汤对湿热内蕴型溃疡性结肠炎大鼠模型结肠组织中AP-1,TNF-α表达的影响

英文题名：Effect of Shaoyaotang on Expressions of AP-1 and TNF-α in Colon of Rats with Hygrothermal Ulcerative Colitis

作者：王凤仪[1];赵党生[1];蒲晓薇[1];祖健[1];徐敏[1];孙晨安[1]

第一作者：王凤仪

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

年份：2019

卷号：25

期号：16

起止页码：7

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金项目(9984011510)

语种：中文

中文关键词：芍药汤;溃疡性结肠炎;激活蛋白-1(AP-1);肿瘤坏死因子-α(TNF-α)

外文关键词：Shaoyaotang;ulcerative colitis;activator protein-1(AP-1);tumour necrosis factor(TNF-α)

摘要：目的:观察芍药汤对湿热内蕴型溃疡性结肠炎(UC)大鼠模型结肠组织中激活蛋白-1(AP-1)与肿瘤坏死因子-α(TNF-α)的基因及蛋白表达的影响,探讨芍药汤治疗UC的作用机制。方法:将60只Wistar大鼠随机分为正常组、模型组、柳氮磺砒啶组及芍药汤低、中、高剂量组,采用病症结合的方法复制湿热内蕴型UC大鼠模型,即高脂高糖辛辣食物及免疫复合法,联合2,4,6-三硝基苯磺酸(TNBS)结合乙醇复合法。造模成功后,分别给予芍药汤低、中、高(6,12,24 g·kg^-1)灌胃,柳氮磺胺嘧啶1 g·kg^-1剂量灌胃,正常组给予等体积生理盐水,连续21 d。实时荧光定量聚合酶链式反应(Real-time PCR)检测结肠组织AP-1,TNF-α mRNA表达,蛋白免疫印迹法(Western blot)检测结肠组织AP-1,TNF-α蛋白表达。结果:与正常组比较,模型组AP-1,TNF-α mRNA及蛋白相对表达量明显升高(P<0.05);与模型组比较,各干预组AP-1,TNF-α mRNA及蛋白表达量明显下降(P<0.05),以芍药高剂量组较为明显。结论:芍药汤可抑制湿热型UC大鼠AP-1蛋白刺激TNF-α的表达,可能是其治疗湿热型UC的机制之一。
Objective: To observe the effect of Shaoyaotang on mRNA and protein expressions of colon tissue activated protein-1( AP-1) and tumor necrosis factor-α( TNF-α) of hot and humid-type intrinsic ulcerative colitis( UC) model in rats,in order to explore the mechanism of action of herbaceous peony decoction in the treatment of UC. Method: Totally 60 Wistar rats were randomly divided into blank group,model group,SASP group,and low,medium and high-dose Shaoyaotang groups. The damp-heat intrinsic UC rat model was replicated based on integrated disease and syndrome,namely,high-fat and high-sugar spicy food and immune complex method combined with 2,4,6-trinitrobenzene sulfolnic acid( TNBS) and ethanol complex method. After the successful modeling,low,medium and high-dose Shaoyaotang( 6,12,24 g·kg^-1) was given by gavage,and 1 g·kg^-1 dose of salazol sulfadiazine was given to by gavage. The blank group was given constant volume normal saline for 21 d.Real-time fluorescence quantitative polymerase chain reaction( Real-time PCR) was used to detect mRNA expressions of AP-1 and TNF-α in colon tissues,and Western blot was used to detect protein expressions of AP-1 and TNF-α in colon tissues. Result: Compared with the blank group,relative mRNA and protein expressions of AP-1,TNF-α in the model group were significantly increased( P < 0. 05). Compared with the model group,mRNA and protein expressions of AP-1,TNF-α in the treatment groups were significantly decreased( P < 0. 05).Conclusion: Shaoyaotang can inhibit the expression of TNF-α and stimulate AP-1 protein expression in rats with damp-heat UC.