文献类型：期刊文献

英文题名：The role of PCBP1 in carbon ion-induced ferroptosis and inhibition of lung adenocarcinoma proliferation

作者：Kang, Zhenchao[1];Fu, Zihan[2];Tian, Xuejiao[2];Geng, Yichao[3];Zhang, Qiuning[3];Liu, Yanli[4];Wang, Xinhua[2];Luo, Hongtao[5];Yang, Zhen[2,3]

第一作者：Kang, Zhenchao

通信作者：Yang, Z[1];Yang, Z[2];Luo, HT[3]

机构：[1]Tumor Hosp Wuwei, Dept Radiotherapy, Wuwei, Peoples R China;[2]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou, Peoples R China;[3]Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China;[4]Tumor Hosp Gansu Prov, Lanzhou, Peoples R China;[5]Gansu Prov Hosp TCM, Lanzhou, Peoples R China

第一机构：Tumor Hosp Wuwei, Dept Radiotherapy, Wuwei, Peoples R China

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou, Peoples R China;[2]corresponding author), Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China;[3]corresponding author), Gansu Prov Hosp TCM, Lanzhou, Peoples R China.|[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;[10735]甘肃中医药大学;

年份：2025

卷号：12

外文期刊名：FRONTIERS IN PUBLIC HEALTH

收录：;WOS:【SSCI(收录号:WOS:001400994500001)，SCI-EXPANDED(收录号:WOS:001400994500001)】；

基金：The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported the Natural Science Foundation of Gansu Province (Project No. 23JRRA1207), the Youth Science and Technology Foundation Project of Gansu Province (23JRRA1730), and the Program of Introducing Talents of Discipline to Universities (No. 2023YJRC-13), and The Science and Technology Project of Lanzhou City, China (No. 2023-1-9).

语种：英文

外文关键词：carbon ion; ferroptosis; PCBP1; lung adenocarcinoma; proliferation

摘要：Objective To investigate the role of PCBP1 in the inhibition of lung adenocarcinoma proliferation by carbon irradiation.Methods A549 cells were irradiated with different doses of carbon ions to observe clonal survival and detect changes in cell proliferation. Whole transcriptome sequencing and the Illumina platform were used to analyze the differentially expressed genes in A549 cells after carbon ion irradiation. The relationship between the expression levels of PCBP1, ACSL4, and ALOX15 and survival was analyzed by combining data from the UCSC database and the Kaplan-Meier Plotter public platform. Additionally, the knockdown of the poly (rC)-binding protein 1 (PCBP1) gene using siRNA techniques was employed to further investigate the relationship between the expression levels of PCBP1 and ALOX15. To investigate the relationship between ALOX15 expression and survival, we assessed changes in key indicators of ferroptosis (mitochondrial morphology, ROS, MDA, and divalent iron) in A549 cells after knocking down the PCBP1 gene using siRNA technology. Additionally, the expressions of PCBP1, ACSL4, and ALOX15 in different groups were further analyzed through RT-PCR and Western blot techniques. The differential expression of PCBP1, ACSL4, and ALOX15 in NSCLC tissues was found to correlate with clinical prognosis for survival.Results Carbon ions significantly inhibited the proliferation of A549 cells, and 5.16 Gy carbon ions significantly induced the expression of differentially expressed genes in these cells. Additionally, carbon ions inhibited the expression of PCBP1, which led to alterations in mitochondrial morphology in lung adenocarcinoma cells. This was associated with a significant increase in the levels of ROS, MDA, and Fe2+. Furthermore, low expression of PCBP1 promoted ferroptosis by increasing the expression of ACSL4 and ALOX15.Conclusion Carbon ions decreased the expression of PCBP1 in A549 cells, and low expression of PCBP1 inhibited tumor proliferation by promoting ferroptosis.