文献类型：期刊文献

中文题名：藏药刺柏提取物对CCl_4致大鼠肝纤维化的保护作用

英文题名：Protective Effect of Juniperus Extract on CCl_4-induced Liver Fibrosis of Rats

作者：武燕[1];何华[1];刘健[2]

第一作者：武燕

机构：[1]甘肃中医药大学,兰州730030;[2]兰州大学第一附属医院,兰州730000

第一机构：甘肃中医药大学

年份：2016

卷号：33

期号：1

起止页码：35

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：甘肃省科技计划项目(1506RJYA039);甘肃中医学院中青年科研基金项目(ZQ2014-6)

语种：中文

中文关键词：刺柏提取物;肝纤维化;氧化应激;MMP-2

外文关键词：Juniperus extract; liver fibrosis; oxidative stress; MMP-2;

摘要：目的研究藏药刺柏提取物(Juniperus extract,JE)对大鼠肝纤维化的保护作用和相关机制。方法利用CCl_4建立大鼠肝纤维化模型,JE(25,50 mg·kg^(-1))连续灌胃给药6周。给药结束后检测血清谷丙转氨酶(ALT)、天冬氨酸氨基转移酶(AST)以及肝组织超氧化物歧化酶(SOD)、丙二醛(MDA)含量;HE染色观察肝组织的变化;应用免疫组化法检测MMP-2的表达。结果与模型组比较,JE组大鼠血清中ALT、AST水平降低(P<0.05),同时肝组织中SOD含量增加(P<0.05或P<0.01),50 mg·kg^(-1) JE组MDA含量降低(P<0.05);病理学研究表明,JE组大鼠肝纤维化程度明显改善,50 mg·kg^(-1) JE组能明显抑制肝组织中MMP-2的表达(P<0.05)。结论 JE可能通过降低MMP-2的表达减少氧化应激,从而改善CCl_4引起的肝纤维化损伤。
OBJECTIVE To explore the effects and possible mechanism of Juniperus extract（JE） on liver fibrosis. METHODS The liver fibrosis model was induced by CCl_4, and JE were administered by gastric perfusion at 25, 50 mg·kg^-1 qd for 6 weeks, at the end of the experiment, the contents of alanine transaminase（ALT）, aspartate aminotransferase（AST）, superoxide dismutase（SOD）, maleic dialdehyde（MDA） and the contents of PINP were measured in liver tissue, the expression of MMP-2 was observed by immunohistochemisty. The pathological changes of liver tissue were examined by HE. RESULTS JE（25, 50 mg·kg^-1） improved the liver function significantly through reducing the level of ALT, AST（P〈0.05）, and increasing the content of SOD（P〈0.05 or 0.01）, 50 mg·kg^-1 JE significantly reduced the centent of MDA and the expression of MMP-2（P〈0.05）. CONCLUSION JE may inhibit the liver fibrosis induced by CCl_4 by decreasing the oxidative stress, the mechanism possibly involves the inhibition of the expression of MMP-2.