文献类型：期刊文献

中文题名：沉默周期蛋白G1基因抑制肺癌细胞A549增殖诱导其凋亡的研究

英文题名：Effects of cyclin G1 gene silencing on proliferation and apoptosis of lung cancer A549 cells

作者：王功臣[1];付兆媛[1];杨斌锋[1];王鑫[1];张志明[1]

第一作者：王功臣

机构：[1]甘肃中医药大学附属医院肿瘤科,兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2018

卷号：35

期号：8

起止页码：1482

中文期刊名：中华实验外科杂志

外文期刊名：Chinese Journal of Experimental Surgery

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

语种：中文

中文关键词：肺癌;周期蛋白G1;细胞增殖;细胞凋亡

外文关键词：Lung cancer;Cyclin G1;Cell proliferation;Cell apoptosis

摘要：目的观察沉默周期蛋白G1（Cyclin G1）基因对肺癌细胞A549增殖及凋亡的影响。方法采用脂质体转染法转染Cyclin G1 siRNA至A549细胞中，使用反转录-聚合酶链反应（RT-PCR）检测A549细胞中Cyclin G1 mRNA表达，Western blot法检测A549细胞中β-连环蛋白（β-catenin）、c-Myc、Cyclin G1蛋白的表达。噻唑蓝（MTT）法检测转染48 h后A549细胞的增殖情况，流式细胞仪检测细胞周期和凋亡率的变化。结果转染48 h后干扰组中细胞的吸光度（A）值、Cyclin G1 mRNA和β-catenin、c-Myc、Cyclin G1蛋白的相对表达水平均显著降低，与对照组比较，差异均有统计学意义（t=14.250，P=0.000；t=10.739，P=0.000）；与对照组比较，干扰组中G0/G1期细胞所占比例和细胞凋亡率均明显升高，而S期和G2/M期细胞比例明显下降，差异均具有统计学意义（t=5.074，P=0.007；t=4.634，P=0.010；t=3.099，P=0.000）。结论沉默Cyclin G1基因能够抑制肺癌细胞增殖，诱导其凋亡，其作用机制可能与阻滞细胞G0/G1期和下调Wnt信号通路中β-catenin、c-Myc蛋白有关。
ObjectiveKnockdown the Cyclin G1 in the A549 cell of lung cancer, to explore its effects on the proliferation and apoptosis of the A549 cell.MethodsCyclin G1 siRNA was transfected into A549 cells by using liposome transfection method, the expression levels of Cyclin G1 mRNA were detected by reverse transcriptase-polymerase chain reaction （RT-PCR）, and the expression levels of β-catenin, c-Myc and Cyclin G1 proteins in A549 cells were detected by Western blotting technique. Thiazole blue methyl thiazol tetrazolium （MTT） method was used to detect the proliferation of A549 cells after 48 h transfection, and the flow cytometry detected changes in cell cycle and apoptosis rate.ResultsThe absorbance value （A） and the expression levels of Cyclin G1 mRNA and β-catenin, c-Myc and Cyclin G1 proteins in A549 of the interference group were significantly lower than those of the control group （t=14.250, P=0.000, t=10.739, P=0.000）. Compared with the control group, the proportion of cells of interference group after 48 h transfection in G0/G1 phaseand apoptosis ratesignificantly increased, proportion of cells in S phaseand G2/M phase significantly decreased, and the differences were statistically significant （t=5.074, P=0.007; t=4.634, P=0.010; t=3.099, P=0.000）.ConclusionKnockdown Cyclin G1 gene can inhibit lung cancer cell proliferation and induce its apoptosis, the mechanism may be related to block the cell cycle in G0/G1 phase and cut β-catenin, c-Myc proteins in Wnt signaling pathways.