文献类型：期刊文献

中文题名：当归多糖防护X线辐射对大鼠肝脏损伤的研究

英文题名：Research on protection of Angelica sinensis polysaccharides on liver injury of rats induced by X-ray radiation

作者：王磊[1];许小敏[1];卢志伟[1];刘永琦[1,2];张利英[1];丁楠[3];何进鹏[3];张艳辉[1];李程豪[1]

第一作者：王磊

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000;[2]敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730000;[3]甘肃省空间辐射生物学重点实验室中国科学院近代物理研究所,甘肃兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2017

卷号：48

期号：20

起止页码：4284

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(81473457)

语种：中文

中文关键词：当归多糖;辐射;肝损伤;核因子E2相关因子2;天冬氨酸转氨酶;丙氨酸转氨酶

外文关键词：Angelica sinensis polysaccharides; radiation; liver damage; Nrf2; AST; ALT

摘要：目的研究当归多糖对X线辐射所致大鼠肝脏损伤的防护作用。方法将40只雄性SD大鼠常规饲养14 d后随机分为对照组,模型组,当归多糖高、中、低剂量(254、127、63.5 mg/kg)组,药物组ig给予不同质量浓度的当归多糖,对照组和模型组ig给予等量蒸馏水,每日1次,每次2 m L,连续给药7 d,从第8天起,除对照组外其余各组大鼠均用X线仪进行全身照射,连续照射2 d,辐射总剂量为6.0 Gy,末次照射后72 h股动脉采血并处死大鼠。检测血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)的活性,以及肝脏组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及过氧化脂(LPO)的量;HE染色观察肝脏组织的形态变化;Western blotting法检测大鼠肝脏组织中核因子E2相关因子2(Nrf2)蛋白的表达情况。结果与对照组比较,模型组大鼠血清中AST、ALT的活性升高,MDA和LPO量明显升高,SOD和GSH-Px活性明显降低,Nrf2蛋白表达上调(P<0.05);HE染色可见肝细胞核缩小、部分消失以及肝细胞浆空泡样变性。与模型组比较,当归多糖高、中、低剂量组大鼠血清中AST、ALT活性降低,肝脏组织中MDA和LPO量降低、SOD和GSH-Px活性增加,Nrf2蛋白表达下调(P<0.05),HE染色可见肝细胞核萎缩以及细胞浆空泡样变性减轻。结论当归多糖对X线辐射导致SD大鼠的肝脏损伤具有保护作用,可能是通过减少肝脏组织中自由基的形成,并提高肝脏组织对自由基的清除能力实现的。
Objective To study the protective role of Angelica sinensis polysaccharides（ASP） on liver injury of SD rat caused by X-ray radiation. Methods Forty SD rats after 14 d of conventional breeding were randomly divided into control group, model group, ASP high-, medium-, and low-dose（254, 127, and 63.5 mg/kg） groups. The intervention groups were given different concentration of ASP lavage, and the control and model groups were given the same amount of distilled water instead, once daily, 2 mL/time, for continuous 7 d. On day 8, except the control group, the rest rats were given X-ray irradiation of total body after 30 min of lavage, for 2 d continuous exposure, and total radiation dose was 6 Gy. After 72 h of irradiation, femoral artery blood was collected and rats were killed. The activities of serum AST, ALT and the contents of SOD, MDA, GSH-Px, and LPO in liver were detected. HE staining was used to observe morphological changes of liver tissue, Western blotting was used to detect the Nrf2 protein expression in rat liver tissue. Results Compared with the blank group, in the model group the activities of AST and ALT in serum of rats, MDA and LPO content increased significantly, SOD and GSH-Px content significantly decreased, and Nrf2 protein expression increased, with statistically significant difference（P〈0.05）. HE staining showed that hepatic nuclei reduces and partly disappears, and liver cells were degenerated. Compared with model group, serum AST and ALT activities of rats in high-, medium-, and low-dose groups decreased, the contents of MDA and LPO decreased, the contents of SOD and GSH-Px in liver tissue increased, and the Nrf2 protein expression decreased, with statistically significant differences（P〈0.05）. HE staining showed hepatic shrinking nucleus and cytoplasm vacuoles degeneration. Conclusion The ASP have protective effect on the liver injury of SD rats caused by X-ray radiation, its mechanism is probably though reducing the formation of free radicals in the liver tissue and improving the liver＇s ability to remove free radicals.