文献类型：期刊文献

英文题名：Low temperature modifies seedling leaf anatomy and gene expression in Hypericum perforatum

作者：Su, Hongyan[1];Jin, Ling[2];Li, Mengfei[1];Pare, Paul W.[3]

第一作者：Su, Hongyan

通信作者：Li, MF[1];Pare, PW[2]

机构：[1]Gansu Agr Univ, State Key Lab Arid Land Crop Sci, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[3]Texas Tech Univ, Dept Chem & Biochem, Lubbuck, TX 79409 USA

第一机构：Gansu Agr Univ, State Key Lab Arid Land Crop Sci, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, State Key Lab Arid Land Crop Sci, Lanzhou, Peoples R China;[2]corresponding author), Texas Tech Univ, Dept Chem & Biochem, Lubbuck, TX 79409 USA.

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85139555036);WOS:【SCI-EXPANDED(收录号:WOS:000874586900001)】；

基金：This research was funded by State Key Laboratory of Aridland Crop Science/Gansu Agricultural University (GSCS-2021-Z03), Assurance Project of Ecological Planting and Quality of Daodi Herbs (202103003).

语种：英文

外文关键词：hypericum perforatum; temperature; green tissue; dark gland; secretory cell; hypericin

摘要：Hypericum perforatum, commonly known as St John's wort, is a perennial herb that produces the anti-depression compounds hypericin (Hyp) and hyperforin. While cool temperatures increase plant growth, Hyp accumulation as well as changes transcript profiles, alterations in leaf structure and genes expression specifically related to Hyp biosynthesis are still unresolved. Here, leaf micro- and ultra-structure is examined, and candidate genes encoding for photosynthesis, energy metabolism and Hyp biosynthesis are reported based on transcriptomic data collected from H. perforatum seedlings grown at 15 and 22 degrees C. Plants grown at a cooler temperature exhibited changes in macro- and micro-leaf anatomy including thicker leaves, an increased number of secretory cell, chloroplasts, mitochondria, starch grains, thylakoid grana, osmiophilic granules and hemispherical droplets. Moreover, genes encoding for photosynthesis (64-genes) and energy (35-genes) as well as Hyp biosynthesis (29-genes) were differentially regulated with an altered growing temperature. The anatomical changes and genes expression are consistent with the plant's ability to accumulate enhanced Hyp levels at low temperatures.