文献类型：期刊文献

英文题名：Electroacupuncture Pretreatment Ameliorates Perioperative Neurocognitive Disorder in Aged Mice by Inhibiting Ferroptosis Through the SIRT1/NRF2/GPX4 Pathway

作者：Du, Zhongying[1,2,3];Zhang, Binsen[4];Chen, Tianren[1];Lei, Chang[1];Tang, Lu[1];Yang, Sasa[1];Wang, Chunai[4,5]

第一作者：Du, Zhongying

通信作者：Wang, CN[1];Wang, CN[2]

机构：[1]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou, Peoples R China;[2]Key Lab Gansu Prov Prescript Min & Innovat Transla, Lanzhou, Peoples R China;[3]Gansu Prov Tradit Chinese Med New Prod Creat Engn, Lanzhou, Peoples R China;[4]Gansu Prov Hosp Tradit Chinese Med, Lanzhou, Peoples R China;[5]Clin Med Res Ctr Integrated Chinese & Western Med, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Prov Hosp Tradit Chinese Med, Lanzhou, Peoples R China;[2]corresponding author), Clin Med Res Ctr Integrated Chinese & Western Med, Lanzhou, Peoples R China.

年份：2026

卷号：30

期号：1

外文期刊名：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

收录：;Scopus(收录号：2-s2.0-105027101161);WOS:【SCI-EXPANDED(收录号:WOS:001661305600001)】；

基金：This work was supported by National Natural Science Foundation of China (Grant 82260973) and Lanzhou Municipal Science and Technolog y (Grant 2022- 3- 31).

语种：英文

外文关键词：electroacupuncture; ferroptosis; perioperative neurocognitive disorder; SIRT1/NRF2/GPX4

摘要：Perioperative neurocognitive disorder (PND) is a common complication after anesthesia surgery in elderly patients, which not only reduces the patients' quality of life but also increases the burden on their families and society. PND has been found to be closely related to ferroptosis. This study investigated whether electroacupuncture (EA) can inhibit ferroptosis through the SIRT1/NRF2/GPX4 pathway to improve PND in aged mice. The PND model was established using sevoflurane anesthesia and tibial fracture surgery. EA was administered at the Baihui (GV 20) and Dazhui (GV 14) acupoints. Additionally, intraperitoneal injection of silent information regulator sirtuin 1 (SIRT1) inhibitor EX527 (5 mg/kg) was administered for five consecutive days before surgery and intraperitoneal injection of ferrostatin-1 (Fer-1) (2 mg/kg) was administered before anesthesia. On the third day after surgery, the cognitive ability of the aged mice was measured using the Y-maze, and motor ability was assessed by total distance in the open field test. Transmission electron microscopy was used to observe hippocampal mitochondrial structure. Immunofluorescence staining was used to detect glutathione peroxidase 4 (GPX4) levels in the hippocampus. Flow cytometry measured ATP content and mitochondrial membrane potential in hippocampal mitochondria. A colorimetric assay was used to detect iron content in hippocampal neurons. Reverse transcription-quantitative polymerase chain reaction and Western blotting were used to detect mRNA and protein expression of Solute carrier family 7 member (SLC7A11), transferrin receptor 1 (TFR1), iron regulatory protein 2 (IRP2), ferritin, SIRT1, nuclear factor erythroid 2-related factor 2 (NRF2) and GPX4. The results showed that compared with the model group, the EA treatment group and the Fer-1 (iron inhibitor) treatment group revealed improved ferroptosis and memory function in hippocampal neurons, while the EX527 (SIRT1 inhibitor) treatment group did not reveal any improvement. In conclusion, the occurrence and progression of PND are closely related to ferroptosis. EA stimulation of the Baihui and Dazhui acupoints can improve PND, possibly by regulating ferroptosis through the SIRT1/NRF2/GPX4 signalling pathway.