文献类型：期刊文献

英文题名：Electroacupuncture Reduces Ocular Surface Neuralgia in Dry-Eyed Guinea Pigs by Inhibiting the Trigeminal Ganglion and Spinal Trigeminal Nucleus Caudalis P2X₃R-PKC Signaling Pathway

作者：Jin, Tuo[1];Liu, Xue[2];Li, Ying[1];Li, Pei-Chen[1];Wan, Mi-Mi[1];Li, Liu-Jiao[1];Shi, Li[1];Fu, Zhang-Yitian[1];Gao, Wei-Ping[1,3]

第一作者：Jin, Tuo

通信作者：Gao, WP[1]

机构：[1]Nanjing Univ Chinese Med, Affiliated Hosp, Dept Ophthalmol, Nanjing, Peoples R China;[2]Gansu Univ Chinese Med, Affiliated Hosp, Dept Ophthalmol, Lanzhou, Peoples R China;[3]Nanjing Univ Chinese Med, Affiliated Hosp, Dept Ophthalmol, Nanjing 210024, Peoples R China

第一机构：Nanjing Univ Chinese Med, Affiliated Hosp, Dept Ophthalmol, Nanjing, Peoples R China

通信机构：[1]corresponding author), Nanjing Univ Chinese Med, Affiliated Hosp, Dept Ophthalmol, Nanjing 210024, Peoples R China.

年份：2023

卷号：48

期号：6

起止页码：546

外文期刊名：CURRENT EYE RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000935797500001)】；

基金：This project was supported by the National Natural Science Foundation of China [No. 82074526, Nanjing, China].

语种：英文

外文关键词：Electroacupuncture; dry eye; ocular surface neuralgiatrigeminal ganglion; spinal trigeminal nucleus caudalis; P2X(3)R-PKC signaling pathway

摘要：PurposeTo observe the effects of electroacupuncture on ocular surface neuralgia and the P2X(3)R-PKC signaling pathway in guinea pigs with dry eye.MethodsA dry eye guinea pig model was established by subcutaneous injection of scopolamine hydrobromide. Guinea pigs were monitored for body weight, palpebral fissure height, number of blinks, corneal fluorescein staining score, phenol red thread test, and corneal mechanical perception threshold. Histopathological changes and mRNA expression of P2X(3)R and protein kinase C in the trigeminal ganglion and spinal trigeminal nucleus caudalis were observed. We performed a second part of the experiment, which involved the P2X(3)R-specific antagonist A317491 and the P2X(3)R agonist ATP in dry-eyed guinea pigs to further validate the involvement of the P2X(3)R-protein kinase C signaling pathway in the regulation of ocular surface neuralgia in dry eye. The number of blinks and corneal mechanical perception threshold were monitored before and 5 min after subconjunctival injection and the protein expression of P2X(3)R and protein kinase C was detected in the trigeminal ganglion and spinal trigeminal nucleus caudalis of guinea pigs.ResultsDry-eyed guinea pigs showed pain-related manifestations and the expression of P2X(3)R and protein kinase C in the trigeminal ganglion and spinal trigeminal nucleus caudalis was upregulated. Electroacupuncture reduced pain-related manifestations and inhibited the expression of P2X(3)R and protein kinase C in the trigeminal ganglion and spinal trigeminal nucleus caudalis. Subconjunctival injection of A317491 attenuated corneal mechanoreceptive nociceptive sensitization in dry-eyed guinea pigs, while ATP blocked the analgesic effect of electroacupuncture.ConclusionsElectroacupuncture reduced ocular surface sensory neuralgia in dry-eyed guinea pigs, and the mechanism of action may be associated with the inhibition of the P2X(3)R-protein kinase C signaling pathway in the trigeminal ganglion and spinal trigeminal nucleus caudalis by electroacupuncture.