文献类型：期刊文献

中文题名：IL-6和TNF-α模拟炎性微环境促进人BMSCs向肿瘤相关成纤维细胞分化

英文题名：IL-6 and TNF-α promote the differentiation of human BMSCs to TFAs by mimicry inflammatory microenvironment

作者：任春贞[1];骆亚莉[1];刘永琦[1,2];李程豪[1];张艳辉[1];王风梅[1];卢志伟[1];李玲[1]

第一作者：任春贞

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室;[2]敦煌医学与转化省部共建教育部重点实验室

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2018

卷号：38

期号：7

起止页码：901

中文期刊名：基础医学与临床

外文期刊名：Basic & Clinical Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金(81360588)

语种：中文

中文关键词：炎性微环境;骨髓间充质干细胞;肿瘤相关成纤维细胞分化

外文关键词：inflammatory microenvironment;BMSCs;TAFs differentiation

摘要：目的观察在IL-6和TNF-α模拟的炎性微环境中骨髓间充质干细胞(BMSCs)是否向肿瘤相关成纤维细胞(TAFs)分化。方法将实验分为9组:对照组、IL-6 50 ng/m L及100 ng/m L干预组、TNF-α50 ng/m L及100 ng/m L干预组、IL-6 50 ng/m L+TNF-α50 ng/m L干预组、IL-6 50 ng/m L+TNF-α100 ng/m L干预组、IL-6 100 ng/m L+TNF-α100 ng/m L干预组、IL-6 100 ng/m L+TNF-α50 ng/m L干预组;连续诱导42 d后,通过倒置相差显微镜、流式细胞计量术及Western blot分别检测BMSCs细胞形态、细胞周期及(TAFs)标记分子α-平滑肌肌动蛋白(α-SMA)、成纤维细胞活化蛋白(FAP)蛋白的表达。结果与对照组相比,IL-6 100 ng/m L+TNF-α50 ng/m L组可明显促进BMSCs细胞增殖;G1期比例降低,S期比例升高,且α-SMA,FAP蛋白表达显著增加(P<0.05)。结论 IL-6 100 ng/m L+TNF-α50 ng/m L所模拟的炎性微环境可诱导BMSCs细胞形态和增殖特性发生异常改变,TAFs标记分子α-SMA和FAP表达量升高。
Objective To observe whether BMSCs differentiate into TAFs in inflammatory microenvironment simulated by IL-6 and TNF-α.Methods The experiment was divided into 9 groups:the BMSCs group,the 50 ng/mL,100 ng/mL IL-6 intervention group,the 50 ng/mL,100 ng/mL TNF-αintervention group,the 50 ng/mL IL-6+50 ng/mL TNF-αintervention group,the 50 ng/mL IL-6+100 ng/mL TNF-αintervention group,the 100 ng/mL IL-6+50 ng/mL TNF-αintervention group and the 100 ng/mL IL-6+100 ng/mL TNF-αintervention group;After continuous induction for 42 days,BMSCs cell morphology and cycle,TAFs-taggedα-SMA and FAP protein were examined by phase-contrast microscope,flow cytometry and Western blot method.Results Compared with the normal control group,the 100 ng/mL IL-6+50 ng/mL TNF-αintervention group BMSC cell proliferation was significantly promoted;G 1 phase decreased in proportion and S phase increased;α-SMA and FAP protein expression was significantly increased(P<0.05).Conclusions Microenvironment simulated by the 100 ng/mL IL-6+50 ng/mL TNF-αmay induce the abnormal change of the BMSCs morphological and proliferative characteristics.TAFs reference moleculeα-SMA and FAP expression is increased.