文献类型：期刊文献

中文题名：党参水提物对D-半乳糖致衰老小鼠肝脾形态结构和Bax蛋白及VEGF表达的影响

英文题名：Effects of the aqueous extract of Codonopsis pilosula on the histolomorph structure and the expression of Bax and VEGF in liver and spleen of aging mouse induced by D-galactose

作者：王晶[1];王勇[1];李海龙[1];杨雅丽[1]

第一作者：王晶

机构：[1]甘肃中医药大学医学技术学院

第一机构：甘肃中医药大学

年份：2016

卷号：52

期号：4

起止页码：72

中文期刊名：西北师范大学学报：自然科学版

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：甘肃省高等学校科研项目(2013A-087);甘肃中医药大学青年教师科研基金资助项目(ZQ2015-20)

语种：中文

中文关键词：党参;衰老;组织形态;Bax;VEGF

外文关键词：Codonopsis pilosula; aging; tissue histolomorph; Bax; VEGF

摘要：为研究党参水提物对D-半乳糖致衰老小鼠肝脏和脾脏组织结构及Bax蛋白及VEGF表达的影响,将100只昆明小鼠随机分为对照组、D-半乳糖致衰老模型组、低、中、高剂量党参干预组.连续处理42d,随时监测小鼠的体重变化,处理结束后,称取脏器质量,观察组织显微结构,并用免疫组织化学法检测Bax蛋白和VEGF在肝脏和脾脏组织的表达.结果显示衰老组小鼠肝脏和脾脏指数明显下降(P<0.05).多数肝细胞水肿,胞浆空淡,呈气球样变.脾窦明显扩张,红髓范围扩大,脾小梁明显增生.Bax蛋白表达明显增强,VEGF蛋白表达显著减少.与衰老组相比,党参干预高剂量组的脏器指数明显增加(P<0.05),肝小叶结构清楚,肝细胞体积增大,肝细胞水肿明显减轻.白髓红髓清楚,红髓范围减小,脾小梁增生不明显.Bax蛋白表达明显减少,VEGF蛋白表达明显增加.表明党参水提物可以稳定肝脾的组织结构,抑制细胞凋亡,促进血管生成,对衰老小鼠的肝脏和脾脏组织有一定的保护作用.
To investigate the effects of Codonopsis pilosula（CPPS）on the liver and spleen histolomorph structure and the expression of Bax and VEGF in liver and spleen of mouse induced by D-galactose,100mouse（Kunming）are divided into normal control group,aging model group and low/middle/high dose of CPPS inerventional groups randomly.After treatment for 42 days,the changes of the liver coefficient and spleen coefficient are recorded,the morphologic changes of these tissues are observed,and the changes in the expression of Bax and VEGF in liver and spleen are also determined by immunohistochemical method.Results show that the liver coefficient and spleen coefficient of aging model group are significantly decreased（P〈0.05）,and markedly swelled,surface is rough and presenting bright red.The positive expression of Bax protein is increased significantly in liver and spleen of aging model group,while positive expression of VEGF protein is decreased significantly（P〈0.05）.Compared with aging model group,the organ coefficients in the high dose of CPPS interventional group are significantly increased（P〈0.05）,the liver swollen is significantly mitigated,and surface is smooth.The positive expression of Bax protein is decreased significantly in liver and spleen of high dose of CPPS interventional group,while the positive expression of VEGF protein is increased significantly（P〈0.05）.All results indicate that CPPS can stabilize the histolomorph structure,inhibit the excessive apoptosis of cells,and promote angiogenesis,which can protect the liver and spleen of aging mouse induced effectively.