文献类型：期刊文献

英文题名：Radiation-specific metabolic reprogramming drives yield-quality balance in Astragalus mongholicus: Comparative insights from gamma-ray and heavy-ion beam irradiation

作者：Wang, Yuanmeng[1,2];Liu, Xiao[1];Wang, Liangcai[2];Li, Xuehu[1,3];Chen, Fanglei[4];Li, Ping[1];Ma, Xiaohui[2,6,7];Wang, Fusheng[5];Li, Li[1,5];Xin, Zhijun[1,3];Lu, Xihong[1,3];Jin, Ling[2,6,7];Zhou, Libin[1,3,8,9]

第一作者：王亚梅;Wang, Yuanmeng;王苑铭

通信作者：Zhou, LB[1];Jin, L[2];Zhou, LB[3];Jin, L[4];Jin, L[5];Zhou, LB[6];Zhou, LB[7]

机构：[1]Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 101408, Peoples R China;[4]State Nucl Secur Technol Ctr, Beijing 102401, Peoples R China;[5]Dingxi Acad Agr Sci, Dingxi 743000, Peoples R China;[6]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China;[7]PRC, MOE, Lanzhou 730000, Peoples R China;[8]Chinese Acad Sci, Inst Modern Phys, State Key Lab Heavy Ion Sci & Technol, Lanzhou 730000, Peoples R China;[9]Kejin Innovat Inst Heavy Ion Beam Biol Ind, Baiyin 730900, Peoples R China

第一机构：Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[3]corresponding author), Univ Chinese Acad Sci, Beijing 101408, Peoples R China;[4]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou 730000, Peoples R China;[5]corresponding author), PRC, MOE, Lanzhou 730000, Peoples R China;[6]corresponding author), Chinese Acad Sci, Inst Modern Phys, State Key Lab Heavy Ion Sci & Technol, Lanzhou 730000, Peoples R China;[7]corresponding author), Kejin Innovat Inst Heavy Ion Beam Biol Ind, Baiyin 730900, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2025

卷号：18

外文期刊名：PLANT STRESS

收录：Scopus(收录号：2-s2.0-105015554246);WOS:【ESCI(收录号:WOS:001583551200002)】；

基金：Financial support provided by the Nuclear Technology R & D Program (HJSYF2024 (31) , China Agriculture Research System of MOF and MARA (CARS-21) , Gansu Provincial Science and Technology Plan Project: Science and Technology Special Touring Special (23CXNA0042) , the Research Program of State Key Laboratory of Heavy Ion Science and Technology, Institute of Modern Physics, Chinese Academy of Sciences (Grant No. HIST2025CS07) , Natural Science Foundation of Gansu Province (22JR5RA119) , Gansu Provincial Key Research and Development Program (25YFNJ001) and Inner Mongolia National Science Foundation (2024ZD23) are gratefully acknowledged.

语种：英文

外文关键词：Astragalus mongholicus; Heavy-ion beam; Gamma-ray; Multi-omics analysis; Flavonoid accumulation

摘要：The inherent trade-off between biomass accumulation and secondary metabolite production remains a fundamental challenge in medicinal plant domestication. While conventional approaches predominantly focus on single-trait improvement, effective strategies for concurrent optimization remain underexplored. Here we present a pioneering comparative investigation employing gamma-ray (GR) and heavy-ion beam (HIB) irradiation (200 Gy) in Astragalus mongholicus cultivation, coupled with integrated transcriptomic and metabolomic profiling to dissect their differential impacts on agronomic traits and medicinal quality. Our findings demonstrate GR irradiation as a potent inducer of biomass enhancement, achieving a remarkable 200% increase in fresh root weight while simultaneously elevating flavonoid content by 20.2%. In comparison, HIB irradiation exerted more pronounced impacts on metabolic reprogramming and genetic regulation despite moderate growth promotion. Multi-omics analysis revealed distinct molecular mechanisms: GR preferentially activated auxin signaling pathways and phenylpropanoid biosynthesis to coordinate root development and flavonoid accumulation, whereas HIB significantly modulated amino acid metabolism and secondary metabolite biosynthesis pathways. This radiation-specific pattern provides unprecedented insights into regulation of secondary metabolic networks regulation. Our study establishes a novel paradigm for radiation-based medicinal plants improvement-GR demonstrates superior potential for biomass and primary bioactive compound enhancement, while HIB offers unique advantages in manipulating complex secondary metabolism. These findings provide a theoretical foundation for precision application of radiation technologies in agronomic strategies aimed at enhancing bioactive compound accumulation in medicinal plants by leveraging the hormesis effect, effectively addressing the persistent yield-quality paradox in pharmaceutical crop cultivation.