文献类型：期刊文献

英文题名：Osthole Attenuates Macrophage Activation in Experimental Asthma by Inhibitingthe NF-B/MIF Signaling Pathway

作者：Li, Ruyi[1];Song, Peng[2,3];Tang, Guofang[4];Wei, Jianghong[4];Rao, Lizong[4];Ma, Libing[4];Jiang, Ming[4];Huang, Jianwei[4];Xu, Qing[4];Wu, Jingjie[4];Lv, Qian[4];Yao, Dong[5];Xiao, Bo[5];Huang, Haiming[5];Lei, Liping[5];Feng, Juntao[1];Mo, Biwen[4]

第一作者：Li, Ruyi

通信作者：Feng, JT[1];Mo, BW[2]

机构：[1]Cent South Univ, Xiangya Hosp, Dept Resp & Crit Care Med, Key Lab Natl Clin Res Ctr Resp Dis, Changsha, Peoples R China;[2]Gansu Univ Chinese Med, Affiliated Hosp, Key Lab Prevent & Treatment Chron Dis Tradit Chin, Lanzhou, Peoples R China;[3]Shanghai Univ Tradit Chinese Med, Innovat Res Inst Tradit Chinese Med, Shanghai, Peoples R China;[4]Guilin Med Univ, Affiliated Hosp, Dept Resp & Crit Care Med, Guilin, Peoples R China;[5]Guilin Med Univ, Lab Pulm Dis, Guilin, Peoples R China

第一机构：Cent South Univ, Xiangya Hosp, Dept Resp & Crit Care Med, Key Lab Natl Clin Res Ctr Resp Dis, Changsha, Peoples R China

通信机构：[1]corresponding author), Cent South Univ, Xiangya Hosp, Dept Resp & Crit Care Med, Key Lab Natl Clin Res Ctr Resp Dis, Changsha, Peoples R China;[2]corresponding author), Guilin Med Univ, Affiliated Hosp, Dept Resp & Crit Care Med, Guilin, Peoples R China.

年份：2021

卷号：12

外文期刊名：FRONTIERS IN PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85103641233);WOS:【SCI-EXPANDED(收录号:WOS:000636735800001)】；

基金：The present study was supported by the National Natural Science Foundation of China (81960007, 81460005, 81760008, and 81803779), Guangxi Natural Science Foundation Program (2015GXNSFAA139107) and the High Level of Innovation Team and Outstanding Scholars Program in Colleges and Universities in Guangxi. Gansu Province Science Foundation for Distinguished Young Scholars (Grant No. 20JR10RA348).

语种：英文

外文关键词：osthole; macrophages; asthma; NF-ĸ B; MIF 46

摘要：Inhibition of activated macrophages is an alternative therapeutic strategy for asthma. We investigated whether a coumarin compound, osthole, isolated from Cnidium monnieri (L.) Cuss, alleviated macrophage activation in vivo and in vitro. Osthole could reduce expression of a marker of activated macrophages, cluster of differentiation (CD)206, in an ovalbumin-challenge model of asthma in mice. Osthole could also inhibit infiltration of inflammatory cells, collagen deposition and production of proinflammatory cytokines [interleukin (IL)-1 beta, tumor necrosis factor-alpha, macrophage migration inhibitory factor (MIF)] in asthmatic mice. In vitro, expression of phosphorylated-I Balpha, MIF and M2 cytokines (Ym-1, Fizz-1, arginase-1) in IL-4-induced macrophages decreased upon exposure to the NF-B inhibitor MG-132. In our short hairpin (sh)RNA-MIF-knockdown model, reduced expression of M2 cytokines was detected in the IL-4 + shRNA-MIF group. Osthole could attenuate the proliferation and migration of an IL-4-induced rat alveolar macrophages line (NR8383). Osthole could reduce IL-4-induced translocation of nuclear factor-kappa B (NF-B) in NR8383 cells. Collectively, our results suggest that osthole ameliorates macrophage activation in asthma by suppressing the NF-B/MIF signaling pathway, and might be a potential agent for treating asthma.