文献类型：期刊文献

中文题名：黄芪神仙定年方对辐射小鼠肝氧化应激损伤的防护作用

英文题名：Protective effects of Astragalus immortal prescription against liver oxidative stress in irradiated mice

作者：冯彩琴[1];刘永琦[1];张利英[1];辛晓明[1];张同同[1];李娟[2];张苡铭[1];陈刚[1];张丽昕[1]

第一作者：冯彩琴

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]甘肃中医药大学附属医院肿瘤科,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2017

卷号：42

期号：12

起止页码：1061

中文期刊名：解放军医学杂志

外文期刊名：Medical Journal of Chinese People's Liberation Army

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：甘肃省重大疾病分子医学与中医药防护重点实验室开放基金(FZYX14-10);敦煌医学与转化省部共建教育部重点实验室开放课题(DHYX14-011)~~

语种：中文

中文关键词：黄芪神仙定年方;辐射;肝脏;氧化应激

外文关键词：Astragatus immortal prescription; radiation; hepatic; oxidative stress

摘要：目的探讨黄芪神仙定年方对X射线辐射后小鼠肝氧化应激损伤的防护作用及可能机制。方法将6~8周龄昆明种小鼠随机分为空白组(NC组)、模型组(IO组)、阳性对照组(TC组)及黄芪神仙定年方低(LH组,4.8mg/kg)、中(MH组,9.6mg/kg)、高(HH组,19.2mg/kg)剂量组,每组10只,分别给予生理盐水、生理盐水、胸腺肽及不同浓度黄芪神仙定年方水煎剂连续灌胃10d,然后采用X射线8Gy全身单次照射,照射后继续灌胃3d,称小鼠体重,观察饮食、饮水量及一般状况。处死小鼠取肝脏,计算肝脏指数;观察肝脏组织形态学变化;测定肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)等氧化应激指标及Nrf2蛋白表达情况。结果与NC组比较,IO组小鼠体重明显降低(P<0.01),病理切片示小鼠肝细胞排列紊乱,中央静脉严重淤血阻塞,其周围肝细胞变性、坏死,肝组织SOD、GSH-Px活性降低,MDA含量升高(P<0.05),Nrf2蛋白表达量明显升高(P<0.01)。与IO组比较,TC组及HH组小鼠体重明显增加(P<0.01),病理切片示肝组织结构基本正常,肝细胞以中央静脉为中心呈放射状排列,肝组织SOD、GSH-Px活性升高,MDA含量降低(P<0.05),Nrf2蛋白表达量明显降低(P<0.01)。结论黄芪神仙定年方对辐射后小鼠肝脏氧化应激损伤具有一定保护作用,其机制可能与抗氧化作用有关。
Objective To explore the protective effect and possible mechanism of Astragalus immortal prescription （ALP） from hepatic oxidative stress in mice irradiated by X ray. Methods Six to eight weeks old Kunming mice were randomly divided into normal control group, irradiation alone group, positive control group, and low, medium and high dose groups of AIP （10 rats in each group）, physiological saline, thymus peptide and different concentration decocta of AIP were given respectively. After continuous gavage for 10 days, the whole body was irradiated with X ray at the dose of 8 Gy at a time, and then continued lavaging for 3 days, with weighing the mice and observing the diet, water intake and general condition. After killing the mice, the liver was take and the liver index was calculated; the morphological changes of the liver tissues were observed and the expressions of superoxide dismutase （SOD）, malondiadehyde （MDA）, glutathione peroxidase （GSH-Px） and nuclear factor erythroid 2-related factor 2 （Yrf2） in the liver tissues were detected. Results Compared with the normal control group, the weight of mice in model group decreased obviously （P〈0.01）; pathological section showed that the liver cells were disordered, the central vein was congested and blocked seriously, the surrounding liver cells were denatured and necrotic; the activity of SOD and GSH-Px in liver tissues decreased, and the content of MDA increased （P〈0.05）, the expression of Nrf2 protein increased significantly （P〈0.01）. Compared with the radiation alone group, the weight of mice in positive control group and high dose AIP group increased significantly （P〈0.01）; pathological section showed that ihe liver tissue structure was basically normal, the liver cells were radially arranged with the central vein as the center; the activity of SOD and GSH-Px in liver tissues increased, and the content of MDA decreased （P〈0.05）, the expression of Nrf2 protein decreased lignificantly （P〈0.01）. Conclusion AIP has protective effect from hepatic oxidative stress in mice irradiated by X ray, and the mechanism may be related to its antioxidant effects.