文献类型：期刊文献

中文题名：归脾汤对苯中毒小鼠骨髓造血干细胞表型Sca-1和CD34＋、细胞分裂周期的影响

英文题名：Influences of Guipi Tang on phenotype Sca-1 and CD34^+ of hematopoietic stem cell and mitotic cycle in benzolism mice

作者：殷丽娟[1];刘立[1];许瑞[1];彭晓明[2];杨欢[1]

第一作者：殷丽娟

机构：[1] 甘肃中医学院,甘肃730000;[2] 甘肃中医学院附属医院

第一机构：甘肃中医药大学

年份：2014

卷号：37

期号：4

起止页码：255

中文期刊名：北京中医药大学学报

外文期刊名：Journal of Beijing University of Traditional Chinese Medicine

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：甘肃省2009年重大中医药科研资助项目(No.GZK2009-08)

语种：中文

中文关键词：归脾汤;苯中毒;造血干细胞;细胞周期;小鼠

外文关键词：Guipi Tang ; benzolism ; hematopoietic stem cell ; cell cycle ; mice

摘要：目的观察归脾汤对苯中毒骨髓造血干细胞表型Sca-1和CD34^+、细胞周期的影响,并探讨其造血调控作用的可能机制。方法将8~12周龄健康昆明种小鼠,雌雄各半,共72只,按随机数字表法随机分为正常对照组、模型组、西药组以及归脾汤高、中、低剂量组,每组12只。将小鼠置于人工模拟高苯浓度(苯浓度=2000 mg/m^3)环境每天8h,连续造模14 d,依据造模标准白细胞水平降至4.5×10~9/L以下,复制苯中毒模型。造模成功后,归脾汤高、中、低剂量组分别给予归脾丸溶液浓度0.072、0.036、0.018 g/mL灌胃,正常对照组和模型组给予生理盐水(20 mL/kg)灌胃,均每天1次,各组均连续给药3周。用全自动血细胞分析仪、流式细胞仪分别检测归脾汤对苯中毒小鼠外周血、骨髓细胞周期、造血干细胞表型Sca-1和CD34^+的影响。结果归脾汤中剂量组和西药组均可激活小鼠骨髓造血干细胞使Sca-1和CD34^+抗原的表达升高,形成更为原始的造血干细胞,从而使其数量增加(P<0.05);大剂量组可促进骨髓G_0/G_1期细胞向S期细胞以及S期细胞向G_2/M期细胞的转化,增殖指数(PI)也明显升高(P<0.05或P<0.01),并能明显升高外周血白细胞(WBC)、血红蛋白(HBG),但对血小板(PLT)的作用不明显。结论归脾汤可能是增加较早期造血干细胞的数量和功能,提高造血能力及通过促使苯中毒小鼠骨髓细胞G_2期检查点,进入细胞增殖周期,加速骨髓G_0/G_1期细胞向S期细胞、S期细胞向G_2/M期细胞的转化,促进骨髓造血细胞增殖,提高外周血象,促进骨髓造血功能的恢复。
Objective To observe the influences of Guipi Tang on phenotype Sca-1 and CD34＋ of hematopoietic stem cell （HSC） and cell cycle in benzolism mice, and discuss its possible mechanism in hematopoietic regulation. Methods Kunming mice （n = 72, 8-12 weeks old, half male and half female） were randomly divided into normal group, model group, Western drug group, and high-dose, mid-dose and low-dose groups （each n = 12）. The benzolism model was established by putting mice into a manual simulated condition with high concentration benzene （ 2000 mg/m3 ） for 8 h a day for 14 d continuously, until the level of WBC dropped below 4.5 × 109/L. The high-dose, mid-dose and low-dosegroups were orally given solution of Guipi Tang （0. 072g/mL, 0. 036g/mL and 0. 018g/mL） respectively and normal group and model group were orally given normal saline water （20 mL/kg） once a day for 3 weeks. The influences of Guipi Tang on mitotic cycle in peripheral blood and bone marrow and phenotype Sca-1 and CD34 ＋ of HSC were detected by using automotive blood cell analyzer and flow cytometry. Results HSC was activated, expressions of Sea-1 and CD34 ＋ increased and primordial HSC increased in mid-dose group and Western drug group （P 〈 0.05）. In high-dose group, the cell transition from G0/G1 phase to S phase and from S phase to G2/M phase were promoted, proliferation index increased significantly （P 〈0.05 or P 〈 0.01 ） , and levels of WBC and HBG increased. The change of platelet was not significant. Conclusion Guipi Tang may improve the quantity and function of early HSC and hemopoietic ability through promoting the cell transition from G0/G1 phase to S phase and from S phase to G2/M phase.