文献类型：期刊文献

中文题名：归芪益元膏对^(12)C^(6+)束辐射损伤模型大鼠旁效应的影响

英文题名：Effect of Guiqiyiyuan ointment on bystander effect in rat models of ^(12)C^(6+) beam radiation damage

作者：梁建庆[1,2];李金田[1,2];李娟[2];刘永琦[2];蔺兴遥[2];刘凤艳[3];安耀荣[2];李晓伟[2];张子同[2]

第一作者：梁建庆

机构：[1]甘肃中医药大学博士后流动站,兰州730000;[2]甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室,兰州730000;[3]甘肃中医药大学附属医院急诊科,兰州730000

第一机构：甘肃中医药大学党委研究生工作部、研究生院

年份：2017

卷号：42

期号：10

起止页码：870

中文期刊名：解放军医学杂志

外文期刊名：Medical Journal of Chinese People's Liberation Army

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金(81460695);中国博士后科学基金第60批面上资助项目(2016M602887)~~

语种：中文

中文关键词：归芪益元膏;辐射损伤;旁效应;氧化应激;DNA甲基化

外文关键词：Guiqiyiyuan ointment; radiation injury; bystander effect; oxidative stress; DNA methylation

摘要：目的探讨^(12)C^(6+)束辐射大鼠的肺-心-肝-脾旁效应损伤及归芪益元膏对其的防治作用及机制。方法选取健康雄性Wistar大鼠90只,随机分为正常对照组(NC组)、单纯辐射组(SR组)、归芪益元膏组(GO组),每组30只。NC组给予2ml生理盐水灌胃,辐射剂量0Gy,SR组给予2ml生理盐水灌胃,辐射剂量8Gy,GO组给予11.83g/kg归芪益元膏灌胃,辐射剂量8Gy,大鼠连续灌胃给药7d,行^(12)C^(6+)束辐射右侧肺部造模。SR组和GO组大鼠造模后48h处死,取心、肝及脾组织。采用比色法测定丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)含量;ELISA法检测DNA甲基化率;免疫组织化学法检测Dnmt1、Dnmt3a、Dnmt3b表达。结果与NC组比较,SR组大鼠SOD、GSH、GSH-Px降低(P<0.01),MDA升高(P<0.01);DNA甲基化水平降低(P<0.01);Dnmt1、Dnmt3a、Dnmt3b表达升高(P<0.01)。与SR组比较,GO组大鼠SOD、GSH、GSH-Px升高(P<0.01),MDA下降(P<0.01);DNA甲基化水平升高(P<0.01);Dnmt1、Dnmt3a、Dnmt3b表达降低(P<0.01)。三组Dnmt1、Dnmt3a、Dnmt3b蛋白均有表达,且分布在心肌细胞、肝细胞、脾脏白髓外周B细胞胞质内。NC组颜色为浅褐色-褐色,呈弱阳性表达;SR组颜色为褐色-棕色,呈强阳性表达;GO组颜色为浅褐色-棕褐色,呈中等度阳性表达。结论归芪益元膏能减轻^(12)C^(6+)束辐射造成的旁效应,其机制与改善氧化应激反应及DNA甲基化水平有关。
Objective To investigate the bystander effect injury to lung-heart-liver-spleen caused by 12 C6＋ beam radiation, and explore the prevention and treatment effect of Guiqiyiyuan ointment on the injur y and its mechanism. Methods Ninety healthy male Wistar rats were randomly divided into 3 groups： NC group（normal control, normal saline 2 ml/kg, n=30）, SR group（simple radiation, 8 Gy, 2 ml/kg, n=30）, GO group（Guiqiyiyuan ointment 11.83 g/kg, radiation, 8 Gy, n=30）. All the rats received intragastric administration for 7 days. The right side of the lung was modeled by 12 C6＋ beam radiation. After modeling, the rats were killed at 48 h. The heart, liver and spleen were taken. The malonaldehyde（MDA）, glutathione（GSH）, glutathione peroxidase（GSH-Px）, superoxide dismutase（SOD） contents were measured by colorimetry, DNA methylation rate was assayed by ELISA, and the expressions of Dnmt1, Dnmt3 a and Dnmt3 b were detected by immunohistochemistry. Results Compared with NC group, the contents of SOD, GSH and GSH-Px decreased（P〈0.01）, MDA increased（P〈0.01）, the level of DNA methylation decreased（P〈0.01）, and the expressions of Dnmt1, Dnmt3 a and Dnmt3 b increased in SR group（P〈0.01）. Compared with SR group, the contents of SOD, GSH and GSH-Px increased（P〈0.01）, MDA decreased（P〈0.01）, the level of DNA methylation increased（P〈0.01）, and the expressions of Dnmt1, Dnmt3 a and Dnmt3 b decreased in GO group（P〈0.01）. Dnmt1, Dnmt3 a and Dnmt3 b proteins were expressed in the cytoplasm of myocardial cells, hepatocytes and peripheral B cells of the white pulp in spleen, in all the groups. The color of NC group was light brown-brown, showing a weak positive expression. The color of SR group was brown-brown, showing a strong positive expression. The color of GO group was light brown-tan, showing a moderate positive expression. Conclusion The Guiqiyiyuan ointment can reduce the bystander effect caused by the 12 C6＋ beam radiation, and its mechanism is related to improving the oxidative stress reaction and the level of DNA methylation.