文献类型：期刊文献

中文题名：维管特异启动子BSP驱动的基因Chi、Glu双价载体构建

英文题名：Constructing Expression Vector of Genes Chi and Glu Drivedby Specific Promoter BSP for Vascular-Specific

作者：刘玲玲[1];车树理[1];禹娟红[1];李润红[1];原霁红[1]

第一作者：刘玲玲

机构：[1]定西师范高等专科学校,甘肃定西743000

第一机构：甘肃中医药大学定西校区

年份：2012

卷号：27

期号：5

起止页码：49

中文期刊名：华北农学报

外文期刊名：Acta Agriculturae Boreali-Sinica

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：甘肃省高等学校研究生导师项目"农杆菌介导的Chi和Glu基因转马铃薯品系的研究(121901)"

语种：中文

中文关键词：双价载体;维管特异启动子;几丁质酶;β-1,3-葡聚糖酶

外文关键词：Dual price vector; Vascular-specific promoter; Chitinase（Chi）; β-1; 3-3-glucanase（Glu）

摘要：几丁质和β-1,3-葡聚糖是绝大多数真菌细胞壁的主要成份,可分别被几丁质酶(Chitinase,Chi)和β-1,3-葡聚糖酶(β-1,3-glucanase,Glu)降解,而且它们具有协同抗真菌的作用,几丁质酶和β-1,3-葡聚糖酶存在于大多数植物细胞中。在正常情况下,植物体内的几丁质酶和β-1,3-葡聚糖酶只有低水平的组成型表达,但在病原菌侵染、诱导物刺激及各种伤害下可诱导产生大量的几丁质酶和β-1,3-葡聚糖酶。而真菌病害是危害植物的主要病害之一,这些病害主要通过韧皮部运输而进一步扩展到其他部位。如果利用维管特异表达启动子调节目的基因在韧皮部高效特异地表达,就可以更为有效地发挥目的基因的作用。为此,克隆了维管特异BSP启动子和Chi、Glu基因,并构建了以BSP驱动的Chi和Glu基因的双价表达载体pBIBCG。重组质粒pBIBCG经过PCR分析鉴定,结果表明,以维管特异启动子BSP驱动的含有双价抗真菌基因的植物重组表达载体已构建成功,为植物抗真菌基因工程育种奠定了基础,对提高植物的整体抗病性具有重要的作用。
Chitin and β-1,3-glucan is the main constituent of most fungal cell walls,which can be degraded by chitinase（Chi） and β-1,3-glucanase（Glu）.Chitinase gene（Chi） and β-1,3-glucan gene（Glu） has a synergistic effect,chitinase and β-1,3-a variety of injuries can be induced in chitin quality enzymes and β-1,3-glucanase exists in most plant cells.In normal circumstances,chitinase and β-1,3-glucanas of the plant body only has low levels of the type of constitutive expression,but in the pathogen infect,induction and a variety of injuries can be induced in chitinase and β-1,3-glucanase.Also,fungal disease is one of main diseases who do harm to plants,these diseases mainly extend to other area through transportation by phloem.If we can use vascular specifically expressed promoter adjustment purpose gene（antifungal disease gene） in the phloem efficient specific to express,it can be more effective to play the role of target gene.A vascular-specific promoter BSP cloned chitinase gene（Chi） and β-1,3-glucanase（Glu） gene,and built a BSP driver Chi and Glu gene the bivalent express carrier pBIBCG.Restructuring the plasmid pBIBCG after restriction enzyme analysis and identification,the results indicate that the vascular-specific start sub-BSP drive containing the bivalent anti-fungal gene plant expression vectors have been constructed successfully.Laid the foundation for genetic engineering breeding for plant anti-fungal,has a very important role to improve the level of overall disease resistance of plants.