文献类型：期刊文献

英文题名：Dopamine-polyethyleneimine co-deposition of a capillary for α-glucosidase immobilization and its application in enzyme inhibitor screening

作者：Li, Peng[1];He, Fu-Qin[1];Ma, Xiao-Hui[2];Jin, Ling[2];Chen, Juan[1]

第一作者：Li, Peng

通信作者：Chen, J[1];Jin, L[2]

机构：[1]Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2021

卷号：42

期号：20

起止页码：2081

外文期刊名：ELECTROPHORESIS

收录：;Scopus(收录号：2-s2.0-85112407142);WOS:【SCI-EXPANDED(收录号:WOS:000685929100001)】；

基金：This work was funded by Special Foundation for National Science and Technology Basic Resources of China (2018FY100701), and the Key Program of Natural Science Foundation of Gansu Province, China (20JR10RA584), National Survey of Traditional Chinese Medicine Resources of National Administration of Traditional Chinese Medicine (GZY-KJS-2018-004; GZY-KJS-2019-001).

语种：英文

外文关键词：Capillary column enzyme reactor; CE; Dopamine-polyethyleneimine co-deposition; alpha-Glucosidase; Inhibitor screening

摘要：An online method based on CE was established to screen alpha-glucosidase inhibitors from traditional Tibetan medicine extracts. First, the inner wall at the inlet of capillary column was simply and effectively functionalized by dopamine-polyethyleneimine co-deposition method, which combines the adhesion property of dopamine and easy cationization of polyethyleneimine. Then alpha-glucosidase was rapidly immobilized on the inner wall of the capillary column by electrostatic adsorption. The inter- and intraday repeatability of the peak area of the enzymatic reaction product (p-nitrophenol) in a capillary was evaluated, and RSD% (n = 3) was 0.94% and 1.09%, respectively. Good batch-to-batch reproducibility of the peak area between different capillaries (RSD = 2.1%, n = 5) shows that the preparation method has good reproducibility. The Michaelis-Menten constant of the immobilized alpha-glucosidase was measured to be 1.18 mM, and the capillary column enzyme reactor retained 85.9% of initial activity after 30 cycles. Finally, it was applied to the screening of enzyme inhibitors in 20 traditional Tibetan medicine extracts. Sixteen medicines with inhibitory activity were screened out, and Rheum australe had the strongest inhibitory effect with an inhibitory rate of 83.3 +/- 0.4%. These results showed that this method is effective to find potential enzyme inhibitors.