文献类型：期刊文献

中文题名：益肺健脾方对急性肺损伤大鼠气道黏液高分泌及EGFR/PKC/NF-κB通路蛋白表达的影响

英文题名：Effects of Yifei Jianpi Prescription on Airway Mucus Hypersecretion and Protein Expressions of EGFR/PKC/NF-κB Pathway in Rats with Acute Lung Injury

作者：杨亚茹[1];杨玉洁[1];朱中博[1];张旭辉[2,3];刘喜平[1];李红梅[2];李世霞[3];周世欣[1]

第一作者：杨亚茹

机构：[1]甘肃中医药大学,甘肃省中药新产品创制工程实验室,甘肃省中医方药挖掘与创新转化重点实验室,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730000;[3]甘肃中医药大学第三附属医院,甘肃白银730900

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2025

卷号：32

期号：9

起止页码：83

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

基金：国家自然科学基金(82260889);甘肃省科技重大专项(22ZD1FA001);第五批全国中医临床优秀人才研修项目(2022年);甘肃省自然科学基金(24JRRD002)。

语种：中文

中文关键词：益肺健脾方;气道黏液高分泌;EGFR/PKC/NF-κB通路;黏蛋白5AC;大鼠

外文关键词：Yifei Jianpi Prescription;airway mucus hypersecretion;EGFR/PKC/NF-κB pathway;MUC5AC;rats

摘要：目的观察益肺健脾方对脂多糖诱导的急性肺损伤(ALI)模型大鼠气道黏液高分泌及EGFR/PKC/NF-κB通路蛋白表达的影响,探讨其治疗ALI的作用机制。方法60只SPF级SD大鼠随机取10只作为空白组,其余大鼠气管内滴注脂多糖建立ALI模型,将造模大鼠随机分为模型组、地塞米松组和益肺健脾方高、中、低剂量组,每组8只。各给药组分别予相应药液灌胃,空白组、模型组予等体积生理盐水灌胃,每日1次,连续14 d。检测大鼠肺功能[最大呼气流速(PEF)、潮气量(TV)、呼气量(EV)、50%呼气流速(EF50)],HE染色观察肺组织形态,AB-PAS染色评估杯状细胞增生及黏液分泌情况,免疫荧光染色检测肺组织表皮生长因子受体(EGFR)、蛋白激酶C(PKC)、核因子-κB(NF-κB)p65、黏蛋白5AC(MUC5AC)蛋白表达,荧光定量PCR检测肺组织EGFR、MUC5AC mRNA表达,ELISA检测肺组织MUC5AC含量。结果与空白组比较,模型组大鼠PEF、TV、EV、EF50显著降低(P<0.01);支气管管壁明显增厚、管腔变窄,肺间质水肿、充血,肺泡壁厚度增加,伴大量炎性细胞浸润,肺组织损伤评分显著升高(P<0.01);杯状细胞增生明显,黏液分泌显著增多(P<0.01);肺组织EGFR、PKC、NF-κB p65、MUC5AC蛋白表达及EGFR、MUC5AC mRNA表达显著升高(P<0.01),肺组织MUC5AC含量显著升高(P<0.01)。与模型组比较,地塞米松组和益肺健脾方各剂量组大鼠PEF、TV、EV、EF50不同程度升高;肺组织病理损伤不同程度减轻,肺组织损伤评分降低;杯状细胞增生减少,黏液分泌减少;肺组织EGFR、PKC、NF-κB p65、MUC5AC蛋白及EGFR、MUC5AC mRNA表达降低,肺组织MUC5AC含量降低,其中地塞米松组和益肺健脾方高、中剂量组差异均有统计学意义(P<0.01)。结论益肺健脾方可抑制脂多糖诱导的ALI模型大鼠气道黏液高分泌及EGFR/PKC/NF-κB通路蛋白高表达,发挥治疗ALI作用。
Objective To observe the effects of Yifei Jianpi Prescription on airway mucus hypersecretion and protein expressions of EGFR/PKC/NF-κB pathway in lipopolysaccharide(LPS)-induced acute lung injury(ALI)model rats;To explore its mechanism in the treatment of ALI.Methods Ten of 60 SPF SD rats were randomly selected as blank group,and the other rats were intratracheal instilled with LPS to establish ALI model.The model rats were randomly divided into model group,dexamethasone group and Yifei Jianpi Prescription high-,medium-and low-dosage groups,with 8 rats in each group.Each treatment group was given corresponding drug solution by gavage,and the blank group and model group were given equal volume of normal saline by gavage,once a day for 14 days.The pulmonary functions of rats were measured[peak expiratory flow(PEF),tidal volume(TV),expiratory volume(EV),50%expiratory flow rate(EF50)],HE staining was used to observe the morphology of lung tissue,AB-PAS staining was used to evaluate the proliferation and mucus secretion of goblet cells,the expressions of epidermal growth factor receptor(EGFR),protein kinase C(PKC),nuclear factor-κB(NF-κB)p65 and MUC5AC in lung tissue were detected by immunofluorescence staining,the mRNA expressions of EGFR and MUC5AC in lung tissue were detected by fluorescent quantitative PCR,and the content of MUC5AC in lung tissue was detected by ELISA.Results Compared with the blank group,PEF,TV,EV and EF50 of the model group rats significantly decreased(P<0.01);the bronchial wall was significantly thickened,the lumen narrowed,pulmonary interstitial edema and hyperemia,the thickness of alveolar wall increased,accompanied by a large number of inflammatory cells infiltration,and the lung tissue injury score increased significantly(P<0.01);goblet cells proliferated significantly,mucus secretion increased significantly(P<0.01);the protein expressions of EGFR,PKC,NF-κB p65,MUC5AC and mRNA expressions of EGFR and MUC5AC in lung tissue increased significantly(P<0.01),and the content of MUC5AC in lung tissue increased significantly(P<0.01).Compared with the model group,PEF,TV,EV and EF50 in dexamethasone group and Yifei Jianpi Prescription each dosage groups increased in varying degrees;the pathological injury of lung tissue was alleviated to varying degrees,the score of lung tissue injury was reduced;the proliferation of goblet cells was reduced,and the secretion of mucus was reduced,the expressions of EGFR,PKC,NF-κB p65,MUC5AC protein and EGFR,MUC5AC mRNA in lung tissue decreased,and the content of MUC5AC in lung tissue decreased.There was statistical significance in dexamethasone group and Yifei Jianpi Prescription high-and medium-dosage groups(P<0.01).Conclusion Yifei Jianpi Prescription can inhibit the hypersecretion of airway mucus and the high expression of EGFR/PKC/NF-κB pathway protein in rats with ALI induced by LPS.