文献类型：期刊文献

英文题名：miR-125b-5p regulates FFA-induced hepatic steatosis in L02 cells by targeting estrogen-related receptor alpha

作者：Gao, Fen[1];Ma, Yanhua[1];Yu, Chun[1];Duan, Qianchen[2]

第一作者：高帆

通信作者：Ma, YH[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[2]Nanchang Univ, Nanchang 330031, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sci Res & Expt Ctr, Lanzhou 730000, Gansu, Peoples R China.|[107359c91a78c5fd2e803]甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）;[10735]甘肃中医药大学;

年份：2025

卷号：959

外文期刊名：GENE

收录：;Scopus(收录号：2-s2.0-105003561095);WOS:【SCI-EXPANDED(收录号:WOS:001483067100001)】；

基金：Funding This work was supported by General Project of Gansu Province Joint Scientific Research Fund (23JRRA1523 to Yanhua Ma) .

语种：英文

外文关键词：miR-125b-5p; Estrogen-related receptor alpha; Free fatty acid; Hepatic steatosis; Nonalcoholic fatty liver disease

摘要：Background & Aims: NAFLD is a global and complex liver disease caused by multiple factors. Intrahepatocellular steatosis is the primary prerequisite for the occurrence and development of NAFLD. It has been shown that miR125b-5p is highly correlated with NAFLD, and ESRRA is a factor that regulates lipid metabolism. The purpose of our study is to investigate whether miR-125b-5p regulates FFA-induced steatosis in L02 cells by targeting ESRRA. Approaches and results: Estrogen-related receptor alpha (ESRRA) was identified as a direct target of miR-125b-5p through database prediction and a dual-luciferase reporter gene assay. L02 cells were induced with free fatty acids (OA:PA, 2:1) at concentrations of 0.3 mM, 0.6 mM, 0.9 mM, 1.2 mM and 1.5 mM for 24 h, 48 h and 72 h, respectively. The degree of hepatocyte steatosis and triglyceride content were separately manifested by oil red O staining and colorimetric method. Cell viability per group was detected by CCK-8 assay. Eventually, 0.9 mM and 24 h were screened out as the optimal concentration and time for establishing the in-vitro model of hepatic steatosis. Followingly, miR-125b-5p and ESRRA were knocked down by transient transfection. We monitored the expressions of lipid metabolism factors SREBP-1c, ACC1 and FAS and determine triglyceride content within the cells per group. The data showed that knockdown of ESRRA led to down-regulation of the expressions of SREBP1, ACC1, FAS and triglyceride content. Meanwhile, knockdown of ESRRA and miR-125b-5p resulted that the expressions of ESRRA, SREBP-1, ACC1, FAS and triglyceride content rebounded. Conclusions: MiR-125b-5p down-regulates the expressions of lipid metabolism-related factors by negatively regulating ESRRA, thereby improving hepatic steatosis.