文献类型：期刊文献

中文题名：电感耦合等离子体质谱法与原子荧光光谱法分析党参不同部位无机元素的动态特征

英文题名：Study on dynamic characteristics of inorganic elements in different parts of Codonopsis pilosula based on ICP-MS and AFS

作者：刘晓玲[1];马海棠[1];王苗[1];李运[2];王行智[2];邱国玉[3];黄钰芳[1];杨扶德[1]

第一作者：刘晓玲

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]兰州市食品药品检验检测研究院,甘肃兰州730050;[3]甘肃药业集团科技创新研究院有限公司,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2025

卷号：40

期号：1

起止页码：75

中文期刊名：华西药学杂志

外文期刊名：West China Journal of Pharmaceutical Sciences

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金资助项目(82160714);国家重点研发计划项目(2018YFC1706305);甘肃省药品科研项目(2022GSMPA0037);甘肃省重点研发计划项目(23YFFA0069);陇原青年创新创业人才项目(2023LQGR49);兰州市科技计划项目(2022-2-2)。

语种：中文

中文关键词：党参;不同部位;微波消解;无机元素;动态特征;电感耦合等离子体质谱法;原子荧光光谱法;质量控制

外文关键词：Codonopsis pilosula;Different parts;Microwave digestion;Inorganic elements;Dynamic characteristic;ICP-MS;AFS;Quality control

摘要：目的采用电感耦合等离子体质谱法(ICP-MS)及原子荧光光谱法(AFS)测定不同生长期党参地上、地下部位中28种无机元素的含量及动态特征。方法样品经微波消解后,采用ICP-MS法测定27种无机元素的含量,AFS法测定Hg元素的含量。并结合聚类热图、相关性、主成分及正交偏最小二乘-判别分析等方法分析其规律。结果28种无机元素在实验浓度范围的线性关系均良好(r^(2)≥0.9973),检测限为1×10^(-4)~5.063μg·L^(-1);精密度、重复性、稳定性试验的RSD均小于4%,平均加样回收率为86.51%~110.88%,RSD为0.16%~6.65%(n=6)。党参地上部位元素的含量在10~11月最高,地下部位的含量在7~11月先降后升。K、Ca、Mg为引起地上、地下部位差异的主要元素。结论所用方法操作简便、灵敏度高,能全面分析党参中无机元素的动态特征,为党参药材的质量控制提供了参考。
OBJECTIVE To determine the contents and dynamic characteristics of 28 inorganic elements in the aboveground and underground parts of Codonopsis pilosula at different growth stages using inductively coupled plasma mass spectrometry(ICP-MS)and atomic fluorescence spectrometry(AFS).METHODS After microwave digestion,the contents of 27 inorganic elements were determined by ICP-MS,and the content of Hg element was determined by AFS.Combined with clustering heat map,correlation,principal components and orthogonal partial least squares-discriminant analysis,the pattern was analyzed.RESULTS The linearity of the 28 inorganic elements was good(r^(2)≥0.9973),and the detection limits ranged from 1×10^(-4)μg·L^(-1)to 5.063μg·L^(-1).The RSDs of the precision,reproducibility,and stability tests were less than 4%.The average spiked recoveries ranged from 86.51%to 110.88%,with the RSDs varying from 0.16%to 6.65%(n=6).The element content of aboveground parts of C.pilosula reached the highest between October and November,while the element content in the underground parts decreased first and then increased from July to November.K,Ca,and Mg were responsible for the differences between the two parts of C.pilosula.CONCLUSION The method is simple to operate and highly sensitive,and can comprehensively analyze the dynamic characteristics of inorganic elements in C.pilosula,providing a reference for the quality control of C.pilosula.