文献类型：期刊文献

中文题名：头穴针刺对局灶性脑缺血大鼠海马旁回炎性反应相关细胞因子表达及脑梗死体积的影响

英文题名：Effect of scalp acupuncture stimulation on cerebral infarct volume and expression of IL-10,IL-6 and IL-1β in para-hippocampal gyrus in acute ischemic cerebrovascular disease rats

作者：王金海[1];张婷卓[2];李兴兰[2];田亮[3];袁博[4];赵敏[1];张振昶[5];杜小正[2];姜华[1]

第一作者：王金海

机构：[1]兰州大学第二医院中医科,兰州730030;[2]甘肃中医药大学针灸推拿学院,兰州730000;[3]甘肃省中医院,兰州730050;[4]甘肃中医药大学附属医院,兰州730000;[5]兰州大学第二医院神经内科,兰州730030

第一机构：兰州大学第二医院中医科,兰州730030

年份：2019

卷号：44

期号：6

起止页码：405

中文期刊名：针刺研究

外文期刊名：Acupuncture Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：兰州大学第二医院院内博士科研基金项目(No.2015-1-26)

语种：中文

中文关键词：头针;缺血性脑血管疾病;白细胞介素-6;白细胞介素-1β;IL-10;脑梗死体积

外文关键词：Scalp acupuncture;Ischemic cerebrovascular disease;IL-6;IL-1β;IL-10;Cerebral infarct volume

摘要：目的:观察头穴针刺干预对局灶性脑缺血大鼠海马旁回白细胞介素(IL)-10、IL-6、IL-1β表达及脑梗死体积的影响,探讨头穴针刺调节急性缺血性脑血管病炎性反应的机制。方法:SD大鼠中随机抽取12只作为正常组,36只采用线栓法建立局灶性脑缺血模型,随机分为模型组、药物组及头针组,每组12只。药物组以吡咯烷二硫代氨基甲酸盐(100mg·kg^-1·d^-1)腹腔注射,1次/d,共7d;头针组给予双侧“顶颞前斜线”快速透刺治疗,留针20min,1次/d,共7d。分别干预刺前和末次干预结束即刻对各组大鼠进行神经功能缺损评分(NDS)和神经行为学评分(NS)。以2,3,5-三苯基氯化四氮唑(TTC)染色法测定各组大鼠脑梗死体积,免疫组织化学法检测各组大鼠海马旁回IL-10、IL-6、IL-1β的表达。结果:与同组干预前比较,药物组与头针组大鼠NDS和NS均显著降低(P<0.01)。与正常组比较,模型组大鼠NDS和NS升高(P<0.01),脑梗死体积明显增大(P<0.01),海马旁回IL-10、IL-6、IL-1β的表达均明显增高(P<0.01);与模型组比较,药物组和头针组大鼠NDS、NS及海马旁回IL-6、IL-1β表达均明显降低(P<0.05),脑梗死体积明显缩小(P<0.05),头针组IL-10表达明显增高(P<0.05)。结论:上调IL-10的表达,抑制IL-6、IL-1β等促炎细胞因子的过度表达,可能是头穴针刺调节缺血性脑血管病炎性反应的机制之一。
Objective To observe the influence of scalp acupuncture on cerebral infarct size and expression of IL-10,IL-6,and IL-1βin the para-hippocampal gyrus in acute ischemic cerebrovascular disease(AICD)rats,so as to investigate its mechanisms underlying improvement of AICD.Methods Forty-eight male SD rats were randomly allocated to normal control(control),AICD model,medication,and scalp acupuncture groups(n=12 per group).The AICD model was established by occlusion of the middle cerebral artery(MCAO).Rats of the medication group received intraperitoneal injection of Ammonium1-Pyrrolidinedithiocarbamate(APDC,100 mg·kg^-1·d^-1),once daily for 7 days.Scalp acupuncture stimulation was applied to bilateral"Dingnieqianxiexian"(MS6)once daily for 7 days.Before and after intervention,the neurologic deficit score(NDS)and the neurological score(NS)were evaluated according to Longa’s and Schabitz’s methods,respectively.At the end of the intervention,the para-hippocampal gyrus and whole brain were collected respectively.The expression levels of IL-10,IL-6 and IL-1βin the para-hippocampal gyrus tissue were detected by immunohistochemistry,and the cerebral infarct volume of the brain was detected by triphenyltetrazollium chloride(TTC)staining after sectioning.Results Following modeling,the NDS,NS and the expression of IL-10,IL-6 and IL-1βin para-hippocampal gyrus were significantly increased in the model group compared with the control group(P<0.01).After the intervention,the NDS,NS and infarct volume,and the expression levels of IL-6 and IL-1βin the para-hippocampal gyrus were significantly decreased in both medication and scalp acupuncture groups compared with the model group(P<0.05),and the expression of IL-10 was further obviously up-regulated in the scalp acupuncture group(P<0.05)rather than in the medication group(P>0.05).The effect of scalp acupuncture was obviously superior to that of medication in up-regulating IL-10 expression level(P<0.05).No significant differences were found between the medication and scalp acupuncture groups in the levels of NDS,NS,infarct volume,IL-6 and IL-1βproteins(P>0.05).Conclusion Scalp acupuncture can improve neurological function and reduce infarct volume in AICD rats,which may be associated with its function in up-regulating the expression of IL-10 and in inhibiting the expression of IL-6 and IL-1βto reduce inflammation reaction.