文献类型：期刊文献

中文题名：艰难梭菌毒素A对K562细胞生长增殖的影响

英文题名：Effects of Clostridium Difficile Toxin A on Proliferation of K562 Cells

作者：李明[1];席亚明[1];陈彻[2];楚惠媛[2];张豪[1];李培[1];邓伟[1]

第一作者：李明

机构：[1]兰州大学第一医院血液科,血液学研究所,甘肃兰州730000;[2]甘肃中医学院科研中心,甘肃兰州730000

第一机构：兰州大学第一医院血液科,血液学研究所,甘肃兰州730000

年份：2011

卷号：19

期号：4

起止页码：894

中文期刊名：中国实验血液学杂志

外文期刊名：Journal of Experimental Hematology

收录：MEDLINE(收录号：21867609);CSTPCD;;Scopus(收录号：2-s2.0-84871951067);CSCD:【CSCD_E2011_2012】；PubMed;

基金：2011年甘肃省卫生行业科研计划管理项目

语种：中文

中文关键词：艰难梭菌毒素A;K562细胞;细胞凋亡

外文关键词：clostridium difficile toxin A; K562 cell; cell apoptosis

摘要：本研究探讨艰难梭菌毒素A(Tcd A)对人慢性髓系白血病(CML)细胞株K562生长增殖的影响及其机制。采用四氮唑蓝比色法(MTT法)观察Tcd A对K562细胞增殖的抑制作用;流式细胞术分析细胞周期及线粒体膜电位的变化;免疫细胞化学法观察细胞色素C蛋白的表达水平;DNA凝胶电泳技术检测细胞凋亡。结果表明,不同浓度的Tcd A明显抑制K562细胞增殖,呈一定的时间和浓度依赖性;流式细胞仪检测显示,细胞阻滞于G0/G1期,并出现凋亡峰,细胞内细胞色素C蛋白含量增高;DNA凝胶电泳出现片段化的梯形带。结论:Tcd A可以抑制K562细胞的生长增殖,并诱导其凋亡,其机制可能与线粒体膜电位降低,基质中释放出致凋亡活性物质细胞色素C有关。
This study was aimed to investigate the effect of clostridium difficile toxin A （ Ted A） on proliferation of K562 cells and its mechanism. The proliferative activity of K562 ceils exposed to Ted A was tested by MTT assay; cell cycle distribution and mitochondrial membrane potential were analyzed by flow cytometry; the protein expression of cytochrome C and DNA fragmentation were observed by immunohistochemistry staining and agarose gel electrophoresis respectively. The results indicated that Ted A inhibited proliferation of K562 cells in a time-and concentration-dependent manner. Cells were arrested at G0/G1 phase. Peak of apoptosis appeared. The protein expression of cytochrome C increased as compared with control group（p 〈 0.05）. Agarose gel electrophoresis of DNA from K562 treated with Ted A revealed a ＂ladder＂ pattern. It is concluded that clostridium difficile toxin A can inhibit proliferation and induce apoptosis of K562 cells. The mechanism may be in relation to decrease of mitochondrial membrane potential and the release of cytochrome C from mitochondria matrix.