文献类型：期刊文献

中文题名：区域差异与生育阶段对党参根际土壤理化性质及微生物多样性的影响

英文题名：Effects of regional differences and growth stages on rhizosphere soil physicochemical properties and microbial diversity of Codonopsis pilosula

作者：曾翠云[1];金智昕[1];陈臻[2];张明成[1];曹嘉昱[1];高佳佳[1];杜弢[1]

第一作者：曾翠云

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]兰州资源环境职业技术大学环境与化工学院,甘肃兰州730021

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2025

期号：12

起止页码：110

中文期刊名：中国土壤与肥料

外文期刊名：Soil and Fertilizer Sciences in China

收录：;北大核心:【北大核心2023】；

基金：兰州资源环境职业技术大学校级重大项目(X2023ZD-01);甘肃省高等学校青年博士基金项目(2023QB-019);兰州市科技计划项目(2023-3-119);甘肃中医药大学2025年研究生创新基金项目(2025CXCY-048)。

语种：中文

中文关键词：党参;根际土壤微生物;土壤理化性质;微生物多样性

外文关键词：Codonopsis pilosula(Franch.)Nannf.;rhizosphere soil microorganisms;soil physicochemical properties;microbial diversity

摘要：对甘肃不同产地党参根际土壤微生物群落组成的差异及其与土壤养分间的相关性规律进行探究,为党参的科学种植提供理论依据。设置陇西、渭源、宕昌3个试验地,测定党参各生育期土壤理化性质,利用高通量测序技术对其根际土壤中的微生物群落组成及差异进行分析,并对土壤理化性质与根际微生物结果进行相关分析。结果表明,宕昌产地的土壤有机质含量为12.25~12.80 g/kg、全氮含量为5.32~6.83 g/kg、全磷含量为0.85~0.87 g/kg、有效磷含量为4.61~6.80 mg/kg,均显著高于陇西和渭源产地(P≤0.05)。陇西产地的真菌群落多样性均高于其他两个产地,宕昌产地的细菌群落多样性显著高于其余两个产地(P≤0.05)。各样本物种群落组成结果表明,优势真菌门为子囊菌门(Ascomycota),优势真菌属为枝孢属(Cladosporium)、镰刀菌属(Fusarium),优势细菌门为假单胞菌门(Pseudomonadota)、酸杆菌门(Acidobacteriota)、放线菌门(Actinomycetota),优势细菌属为Gp6、鞘氨醇单胞菌属(Sphingomonas)。相关分析显示,真菌群落中,收获期子囊菌门与pH、速效钾呈极显著正相关(P≤0.01),与有机质、全氮、全磷、全钾呈极显著负相关(P≤0.01),与有效磷呈显著负相关(P≤0.05)。细菌群落中,生长旺盛期假单胞菌门与水分呈显著正相关(P≤0.05),与碱解氮呈显著负相关(P≤0.05);酸杆菌门与pH、水分、速效钾呈极显著负相关(P≤0.01),与有机质、全氮、碱解氮、有效磷呈极显著正相关(P≤0.01);放线菌门与全磷、全钾呈极显著负相关(P≤0.01)。冗余分析结果表明,pH、全磷、有效磷是显著影响党参根际优势菌群落组成的土壤理化因子。综上,党参根际微生物物种组成随生育期发生改变,真菌及细菌群落多样性在齐苗期较高;各产地在优势真菌门、属组成上大多一致,但所占丰度不一,土壤理化因子显著调控微生物群落结构,本研究为党参种植的土壤改良和微生物调控提供了理论依据。
The difference of microbial community composition in the rhizosphere soil of Codonopsis pilosula in different producing areas of Gansu province and its correlation with soil nutrients were explored to provide a theoretical basis for the scientific planting of C.pilosula.In this study,three experimental sites of Longxi,Weiyuan and Tanchang were set up to determine the physical and chemical properties of the soil in each growth period of C.pilosula.The composition and difference of microbial communities in the rhizosphere soil were analyzed by high-throughput sequencing technology,and the correlation between soil physical and chemical properties and rhizosphere microbial results was analyzed.The results showed that the contents of soil organic matter(SOM),total nitrogen(TN),total phosphorus(TP)and available phosphorus(AP)in the soil of Tanchang were 12.25-12.80 g/kg,5.32-6.83 g/kg,0.85-0.87 g/kg and 4.61-6.80 mg/kg,respectively,which were significantly higher than those in Longxi and Weiyuan(P≤0.05).The diversity of fungal community in Longxi was higher than that in the other two producing areas,and the diversity of bacterial community in Tanchang was significantly higher than that in the other two producing areas(P≤0.05).The results of community composition of each sample showed that the dominant fungal phylum was Ascomycota,the dominant fungal genera were Cladosporium and Fusarium,the dominant bacterial phylum was Pseudomonadota,Acidobacteriota and Actinomycetota,and the dominant bacterial genera were Gp6 and Sphingomonas.Correlation analysis showed that Ascomycota was significantly positively correlated with pH and aavailable potassium(AK)(P≤0.01),significantly negatively correlated with SOM,TN,TP and total potassium(TK)(P≤0.01),and significantly negatively correlated with AP(P≤0.05).In the bacterial community,Pseudomonadota was positively correlated with soil water content(SWC)(P≤0.05)and negatively correlated with alkaline hydrolysis nitrogen(AN)(P≤0.05).Acidobacteriota was significantly negatively correlated with pH,SWC and AK(P≤0.01),and significantly positively correlated with SOM,TN,AN and AP(P≤0.01).Actinomycetota was significantly negatively correlated with TP and TK(P≤0.01).The results of redundancy analysis showed that pH,TP and AP were the soil physical and chemical factors that significantly affected the community composition of dominant bacteria in the rhizosphere of C.pilosula.In summary,the microbial species composition of C.pilosula rhizosphere changed with the growth period,and the diversity of fungal and bacterial communities was higher at the seedling stage.The composition of dominant fungal phylum and genera was mostly consistent in different habitats,but the relative abundance was different.Soil physical and chemical factors significantly regulated the microbial community structure.This study provided a theoretical basis for soil improvement and microbial regulation of C.pilosula planting.