文献类型：期刊文献

中文题名：Efficacy of Cigu Xiaozhi pill(慈菇消脂丸) on non-alcoholic steatohepatitis-associated lipoapoptosis through stress-activated c-Jun N-terminal kinase signalling pathway

作者：MA Yanhua[1];YU Chengzu[1];WU Yan[1];HAN Tao[2];YANG Shaojun[3];SHI Xia[4]

第一作者：马燕花

机构：[1]College of Clinical Medicine,Gansu University of Chinese Medicine,Lanzhou 730000,China;[2]College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China;[3]Guangxi Beihai Hospital of Traditional Chinese Medicine,Beihai 536000,China;[4]Center of Teaching experiment,Gansu University of Chinese Medicine,Lanzhou 730000,China

第一机构：甘肃中医药大学

年份：2021

卷号：41

期号：1

起止页码：79

中文期刊名：Journal of Traditional Chinese Medicine

外文期刊名：中医杂志（英文版）

收录：Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：Supported by the Regional Fund Project of National Natural Science Foundation of China (No. 81560753): the Effect of JNK/C-Jun Signaling Pathway on Fas Mediated Lipogenic Apoptosis of Stem Cells in Non-Alcoholic Steatohepatitis and the Intervention of Detoxification。

语种：中文

中文关键词：Non-alcoholic fatty liver disease;JNK mitogen-activated protein kinases;apoptosis;stress-activated signaling pathway;Cigu Xiaozhi pill

摘要：OBJECTIVE: To investigate the efficacy of Cigu Xiaozhi pill(慈菇消脂丸, CGXZ) on non-alcoholic steatohepatitis(NASH)-associated lipoapoptosis through the stress-activated c-Jun N-terminal kinase(JNK)/stress-activated protein kinase signalling pathway.METHODS: Sixty male Sprague-Dawley rats were randomly divided into the following groups(10 rats each): blank control, model, low-dose CGXZ,medium-dose CGXZ, high-dose CGXZ, and positive control(treated with SP600125, a JNK inhibitor).The NASH model was established and the histomor-phological characteristics of haematoxylin and eosin-stained liver tissues were examined under a light microscope. Cell apoptosis in liver tissues was assessed via terminal deoxynucleotidyl transferase d UTP nick-end labelling assay. In addition, the m RNA and protein expression levels of p-JNK,p-c-Jun, caspase-8, Fas, and Fas-L were determined via fluorescence-based quantitative real-time PCR,immunohistochemical and Western blot assays.RESULTS: Histopathological examination of the liver showed that the model rats had moderate-to-severe steatosis with infiltration of inflammatory cells as well as significantly higher expression levels of the p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L proteins, compared with those in the blank control group(P < 0.01). Hepatic lobules of the rats in the treatment groups showed significantly reduced vacuolar degeneration and steatosis as well as alleviated inflammatory cell infiltration. The high and medium-dose CGXZ groups exhibited significantly lower m RNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, compared with those in the model group(P < 0.05 or P <0.01).CONCLUSION: CGXZ pill inhibited the onset of hepatocyte apoptosis by regulating the expression of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, thereby exerting therapeutic effects against NASH.