文献类型：期刊文献

英文题名：Toxic effects of realgar transforming solution on the reproduction of Caenorhabditis elegans

作者：Liu, Dongling[1,2,3];Zhi, Dejuan[4];Wang, Mengqi[1];Bai, Yanli[1];Gao, Chunhong[4];Gao, Jiangli[1];Tian, Jing[4];Wang, Dong[1];Li, Hongyu[1]

第一作者：Liu, Dongling;刘东玲

通信作者：Li, HY[1]

机构：[1]Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environ, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Gansu Key Lab Tradit Chinese Med Qual & Stand, Lanzhou, Gansu, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Key Lab Pharmacol & Toxicol Tradit Chinese Med Ga, Lanzhou, Gansu, Peoples R China;[4]Lanzhou Univ, Sch Pharmaceut, Microbial & Biochem Pharm, Lanzhou, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environ, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environ, Lanzhou, Gansu, Peoples R China.

年份：2018

卷号：100

期号：1

起止页码：54

外文期刊名：TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000428058900006)】；

基金：This work was supported by the Sub-Project of National Science and Technology Major Projects for " Major New Drugs Innovation and Development" [grant number 2015ZX09501-004-003-008]; National Natural Science Foundation of China [grant number 81603407], [grant number 81760789]; Technology Program of Gansu Province [grant number 2014A-078].

语种：英文

外文关键词：Realgar transforming solution; reproduction; reproductive toxicity; MAPK pathway; p53 pathway

摘要：Realgar transforming solution is an arsenic formulation which has shown anticancer effects with low toxicity both in vivo and in vitro. In this study, Caenorhabditis elegans was used to evaluate its reproductive toxicity and its possible mechanisms. Realgar transforming solution decreased the brood size and induced proliferation arrest and apoptosis significantly only at an elemental concentration of 37.5mg/L, while arsenic trioxide reduced the brood sizes and induced proliferation arrest and apoptosis of both the wild type N2 and let-60 ras(gf) mutant worms in an arsenic concentration dependent manner. Mitogen-activated protein kinase and protein p53 pathways may be involved in reproductive toxicity as evidenced by real-time quantitative polymerase chain reaction, RNA interference, and inhibition experiments with mitogen-activated protein kinases and p53. In conclusion, realgar transforming solution at the low arsenic (As) concentrations showed lower reproductive toxicity than arsenic trioxide, and a different molecular mechanism of reproductive toxicity is suggested.