文献类型：期刊文献

中文题名：甘肃不同产地红芪中总黄酮及总多糖含量测定研究

英文题名：Content Determination of Total Flavones and Polysaccharides in Hedysari Radix in Different Producing Areas in Gansu Province

作者：杨秀娟[1];杨志军[1,2];牛鹏贤[1];潘子昱[1];邵晶[1];李硕[1,2]

第一作者：杨秀娟

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：25

期号：2

起止页码：79

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：甘肃省教育厅高等学校科研项目(2016B-055;2016B-054;2017A-056);甘肃省财政厅高等学校基本科研业务费项目(2013-1)

语种：中文

中文关键词：红芪;紫外分光光度法;毛蕊异黄酮苷;葡萄糖;总黄酮;总多糖

外文关键词：Hedysari Radix; ultraviolet spectroscopy; calycosin-7-glucoside; glucose; total flavones; total polysaccharides

摘要：目的建立甘肃不同产地红芪中总黄酮及总多糖的含量测定方法。方法采用紫外分光光度法,以毛蕊异黄酮葡萄糖苷、葡萄糖为对照品,在波长分别为260、484 nm处测定甘肃不同产地红芪中总黄酮和总多糖的含量。结果甘肃不同产地红芪中总黄酮含量在2.82~6.79 mg/g之间,平均加样回收率为97.96%;总多糖含量在106.14~746.40 mg/g之间,加样回收率为102.90%。结论甘肃不同产地红芪中总黄酮、总多糖含量存在差异,采用紫外分光光度法测定红芪中总黄酮、总多糖含量的方法操作简便,重复性好,准确可靠,可作为红芪中总黄酮和总多糖的测定方法。
Objective To establish a method for content determination of total flavones and polysaccharides in Hedysari Radix in different producing areas in Gansu Province. Methods The contents of total flavones and polysaccharides in Hedysari Radix in different producing areas in Gansu Province were determined by ultraviolet spectroscopy with calycosin-7-glucoside and glucose as reference substance, and the wavelength was set at 260 nm and 484 nm. Results The contents were from 2.82 mg/g to 6.79 mg/g for total flavones and from 106.14 mg/g to 746.40 mg/g for total polysaccharides in Hedysari Radix in different producing areas in Gansu Province. The recoveries of total flavones and total polysaccharides were 97.96% and 102.90%, respectively. Conclusion There was difference in contents of total flavones and polysaccharides of Hedysari Radix in different producing areas in Gansu Province, and the method of using ultraviolet spectroscopy is simple, reproducible, accurate and reliable, which can be preferably used as the method for content determination of total flavones and polysaccharides in Hedysari Radix.