文献类型：期刊文献

中文题名：基于网络药理学的党参干预肠易激综合征作用机制研究

英文题名：Study on Mechanism of Codonopsis Radix in Treatment of Irritable Bowel Syndrome Based on Network Pharmacology

作者：蒙洁[1];陈冬梅[1];刘佳佳[1];李丹丹[1];王晶[1]

第一作者：蒙洁

机构：[1]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2020

卷号：27

期号：1

起止页码：80

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金(81760835);甘肃省高等学校科学研究一般项目(2018A-052)。

语种：中文

中文关键词：党参;肠易激综合征;免疫反应;炎症因子;网络药理学

外文关键词：Codonopsis Radix;irritable bowel syndrome;immune reaction;inflammatory factor;network pharmacology

摘要：目的基于网络药理学探讨党参治疗肠易激综合征的作用靶点和作用机制。方法通过中药系统药理学数据库与分析平台(TCMSP)筛选出党参口服生物利用度≥30%和类药性≥0.18的化合物作为候选药效成分,利用HTDocking平台预测与候选药效成分匹配的靶蛋白,采用Cytoscape3.7.0软件构建化合物-靶点网络。通过GeneCards、NCBI和OMIM数据库查找肠易激综合征相关基因,利用Cytoscape3.7.0绘制疾病基因蛋白相互作用网络,并与党参化合物-靶点网络进行映射,发现党参治疗肠易激综合征的关键化合物和潜在作用靶点。使用Funrich3.1.3软件对潜在作用靶点进行GO功能注释和KEGG通路富集,对党参治疗肠易激综合征的作用机制做出合理推测。结果共得到党参候选药效成分17个,预测作用靶点111个,肠易激综合征相关基因1368个,党参治疗肠易激综合征的关键化合物15个,潜在作用靶点55个(包括IL6、PTGS2、AKT1、EGFR、IL2、IL10、IL4等),靶点参与的功能主要有免疫应答、炎症反应等,主要富集通路有TRAILA信号通路、TNF信号通路等448条。结论党参可能通过调节PTGS2等炎症因子,参与TRAIL信号通路、TNF信号通路等途径,调节免疫反应,从而达到治疗肠易激综合征的目的。
Objective To explore the targets and mechanism of Codonopsis Radix in the treatment of irritable bowel syndrome(IBS)based on network pharmacology.Methods Compounds with oral bioavailability of 30%or more and drug-like ratio of 0.18 or higher in Codonopsis Radix were screened as candidate drug-effect components by TCMSP database,and then the biomacromolecules matched with candidate drug-effect components were extracted by HTDocking platform.The compound-target interaction network was constructed by Cytoscape 3.7.0.The genes for IBS was searched by GeneCards,NCBI and OMIM,and the protein interaction(PPI)network was mapped using cytoscape 3.7.0,and mapping with the compound-target network of Codonopsis Radix revealed the key compounds and potential targets of Codonopsis Radix in the treatment of irritable bowel syndrome.Funrich 3.1.3 software was used to perform GO function annotation and KEGG pathway enrichment on potential targets,and made a reasonable speculation on the mechanism of Codonopsis Radix in the treatment of IBS.Results Totally 17 candidate pharmacodynamic components,111 predicted targets,and 1368 genes related to IBS were identified.15 key components of irritable bowel syndrome.There were 55 potential targets,including IL6,PTGS2,AKT1,EGFR,IL2,IL10,IL4,etc.;the main functions involved in the targets were immune response,inflammatory response,etc.,and the main enrichment pathways included TRAILA signaling pathway and TNF signaling pathway.Conclusion Codonopsis Radix participates in the TRAIL signaling pathway,TNF signaling pathway,etc.,and regulates the immune response through the regulation of inflammatory factors such as PTGS2,so as to achieve the purpose of treating IBS.