文献类型：期刊文献

中文题名：热补针法对寒证类风湿关节炎模型家兔滑膜组织代谢物谱的影响

英文题名：Metabonomics study on synovial tissues of rheumatoid arthritis cold syndrome rabbit treated with heat-reinforcing needling

作者：袁博[1];王金海[2];方晓丽[1];张星华[1];田亮[1];张婷卓[1];李兴兰[1];杜小正[1];曾华宗[3]

第一作者：袁博

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]兰州大学第二医院;[3]上海聚类生物科技有限公司

第一机构：甘肃中医药大学

年份：2017

卷号：37

期号：4

起止页码：800

中文期刊名：中国老年学杂志

外文期刊名：Chinese Journal of Gerontology

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：国家自然科学基金(81260558);国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(2305135901);甘肃省自然科学基金(1208RJZA185);兰州市科技计划项目(2014-1-247)

语种：中文

中文关键词：热补针法;类风湿关节炎;滑膜组织;代谢组学;液相色谱-质谱

外文关键词：Heat-reinforcing needling; Rheumatoid arthritis; Synovial tissue; Metabonomics; Liquid chromatography-mass spectrometry

摘要：目的探讨比较不同针法对寒证类风湿关节炎(RA)模型家兔滑膜组织代谢物谱的影响及热补针法治疗RA的特异性机制。方法 40只清洁级青紫蓝家兔随机分为正常组、模型组、平补平泻组、捻转补法组、热补针法组,每组8只。以卵蛋白诱导联合低温冷冻法建立寒证RA模型。除正常组、模型组外,其余各组分别于足三里穴施以平补平泻、捻转补法、热补针法针刺,1次/d,留针30 min,共7次。治疗结束后分离家兔膝关节滑膜组织,采用液相色谱-四级杆飞行时间质谱(LC-Q/TOF-MS)检测滑膜组织代谢物,利用主成分分析(PCA)和正交偏最小二乘方判别分析(OPLS-DA)方法对数据进行统计分析。结果与正常组比较,模型组滑膜组织代谢物变化主要体现于丙氨酸、瓜氨酸、脯氨酸、谷氨酰胺、甘氨酸、谷氨酸、鞘氨醇1-磷酸、溶血卵磷脂、肌酸、异亮氨酸/亮氨酸、缬氨酸含量降低(P<0.05);与模型组比较,各针刺组上述代谢物含量增高(P<0.05),且热补针法组肌酸、异亮氨酸/亮氨酸、缬氨酸含量明显高于平补平泻组和捻转补法组。结论热补针法通过调节与RA能量代谢相关的氨基酸代谢,在改善RA机体氨基酸的供给及减缓负氮平衡方面有一定的特异性作用。
Objective To compare the influence of metabolites in synovial tissues of rheumatoid arthritis（ RA） cold syndrome rabbits treated with different needling and investigate the specificity mechanisms of heat-reinforcing needling for RA. Methods A total of 40 healthy purple blue rabbits were randomly divided into normal control（ NC）,model control（ MC）,reinforcing-reducing needling group（ RRN）,twirling-reinforcing needling group（ TRN） and heat-reinforcing needling group（ HRN）（ n = 8）. RA cold syndrome rabbit model was made with ovalbumin and freezing. Except NC and MC,RRN was given acupuncture of reinforcing-reducing needling at Zusanli（ ST36）,TRN was given acupuncture of twirling-reinforcing needling at Zusanli（ ST36）,and HRN was administrated acupuncture of heat-reinforcing needling at Zusanli（ ST36）,once a day and retaining 30 min,for 7 times. Fresh synovial tissues of rabbit knee joints were extracted after the intervention,then liquid chromatography quadrupole time-of-flight mass spectrometry（ LC-Q / TOF-MS） technology was used to evaluate metabolic profiles. All the data were analyzed by principal component analysis（ PCA） and orthogonal partial least squares discriminant analysis（ OPLS-DA） by using the SIMCA-P（ 13.0） software for windows. The results were compared. Results The synovial tissues metabolites concerning alanine,citrulline,proline,glutamine,glycine,glutamic acid,sphingolin 1-phosphoric acid,lysoph-osphatidylcholine,creatine,isoleucine,leucine,and valine in MC mainly were decreased compared with NC（ P 0.05）. The above-mentioned metabolites were increased compared with MC and all acupuncture groups（ P〈0.05）,but the metabolites concerning creatine,isoleucine,leucine,valine in HRN were obviously increased than those of RRN and TRN（ P〈0.05）. Conclusions By adjusting the amino acids metabolism which is associated with RA energy metabolism,heat-reinforcing needling has specific effect on RA by improving supply of amino acids and relieving negative nitrogen balance.