文献类型：期刊文献

中文题名：党参丛生芽的诱导和增殖培养

英文题名：Studies on primary culture and subculture of Codonopsis pilosula(Franch.)Nannf.

作者：张延红[1];郭清毅[1];高素芳[1];陈红刚[1];王惠珍[1];何春雨[1]

第一作者：张延红

机构：[1]甘肃中医药大学药学院,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2020

卷号：31

期号：8

起止页码：1978

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金(81760683);甘肃省高等学校产业支撑计划项目(2020C-09)。

语种：中文

中文关键词：党参;初代培养;继代培养;激素;腋芽;愈伤组织;丛生芽

外文关键词：Codonopsis pilosula(Franch.)Nannf.;Primary culture;Subculture;Plant hormone;Axillary buds;Callus;Cluster buds

摘要：目的筛选出适于诱导党参愈伤组织、丛生芽的最佳激素浓度配比。方法取二年生带腋芽的党参茎段作外植体,MS为基本培养基,添加不同浓度配比的6-BA和NAA,研究外植体的愈伤组织和芽的诱导率和生长势。结果适宜诱导愈伤组织的培养基为:MS+6-BA 2.0 mg/L+NAA 1.5 mg/L和MS+6-BA1.0 mg/L+NAA0.5 mg/L,适宜丛生芽诱导和继代的培养基为:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L和MS+6-BA 0.1 mg/L+NAA 0.1 mg/L;6-BA对丛生芽的生长势影响显著,一般低浓度的6-BA配合使用低浓度的NAA有利于丛生芽的诱导和生长。结论该试验为党参种苗的工厂化快速繁殖及基因工程与遗传转化奠定了良好的技术基础。
Objective Induction of callus and cluster buds of Codonopsis pilosula(Franch.)Nannf. were studied.Methods Based on MS medium, stems with axillary buds were in oculated in mediums containing NAA and 6-BA two regulators. Results A good medium for inducing callus is MS + 6-BA 2.0 mg/L + NAA 1.5 mg/l and MS+ 6-BA 1.0 mg/L + NAA 0.5 mg/L;MS+ 6-BA 0.5 mg/L + NAA 0.1 mg/L and MS+6-BA 0.1 mg/L + NAA 0.1 mg/L are good for bud induction. Low concentration of 6-BA and NAA can induce a high ratio of callus. The concentration of 6-BA obviously influences the ratio of bud differentiation. Conclusion To provide scientific basis for rapid production and gene engineering.